Adenoviruses isolated from civilian and military personnel in the city of Rio de Janeiro, Brazil

Adenovirus are important pathogen primarily associated to respiratory infections of children and military personnel, even though it is also associated to cases of conjunctivitis and keratoconjunctivitis. We analyzed respiratory secretion collected from subjects with and without respiratory infection...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2003-08, Vol.45 (4), p.233-236
Main Authors: de Albuquerque, Maria Carolina Maciel, da Silva, Fabiano Murga, Soares, Caroline Cordeiro, Volotão, Eduardo de Mello, Santos, Norma
Format: Article
Language:English
Subjects:
Adenovirus
Adenovirus Infections, Human - epidemiology
Adenovirus Infections, Human - virology
Adenoviruses, Human - classification
Brazil - epidemiology
Cytopathogenic Effect, Viral
Epidemiology
Humans
Incidence
Military Personnel
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Respiratory infections
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - virology
TROPICAL MEDICINE
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Summary:Adenovirus are important pathogen primarily associated to respiratory infections of children and military personnel, even though it is also associated to cases of conjunctivitis and keratoconjunctivitis. We analyzed respiratory secretion collected from subjects with and without respiratory infection symptoms, being 181 civilians and 221 military subjects. The samples were inoculated in HEp-2 and/or A549 tissue cultures for viral isolation. Samples presenting cytopathogenic effect (CPE) in any tissue culture were tested by a polymerase chain reaction (PCR) assay to confirm adenovirus isolation. The isolates confirmed as adenovirus were further analyzed by restriction endonuclease assay for determination of viral species. Three isolates were identified as specie A (two from civilian and one from military), one isolate from military was identified as specie C, and one isolate from civilian was identified as specie D. For two isolates the specie could not be identified.
ISSN:0036-4665
1678-9946
0036-4665
1678-9946
DOI:10.1590/s0036-46652003000400012