Pimarane-Type Diterpenes with Anti-Inflammatory Activity from Arctic-Derived Fungus Eutypella sp. D-1

The Arctic-derived fungus Eutypella sp. D-1 can produce numerous secondary metabolites, and some compounds exhibit excellent biological activity. Seven pimarane-type diterpenes, including three new compounds eutypellenone F (1), libertellenone Y (2), and libertellenone Z (3), and four known compound...

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Published in:Marine drugs 2023-10, Vol.21 (10), p.541
Main Authors: Ning, Yaodong, Zhang, Shi, Zheng, Te, Xu, Yao, Li, Song, Zhang, Jianpeng, Jiao, Binghua, Zhang, Yun, Ma, Zengling, Lu, Xiaoling
Format: Article
Language:English
Subjects:
anti-inflammatory
Anti-inflammatory agents
Biological activity
Carbon
Cell culture
Cell migration
Culture media
diterpene
Diterpenes
Ethanol
Eutypella sp. D-1
Fermentation
Fungi
Inflammation
Metabolites
Migrations
Nitric oxide
Polar environments
Secondary metabolites
Spectrum analysis
Tetrahydrofuran
Zebrafish
zebrafish model
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cited_by cdi_FETCH-LOGICAL-c450t-519de0d61fe6fab721e13554c3a903655a648c97ec2714233e13c07119ed7f0a3
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container_issue 10
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container_title Marine drugs
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creator Ning, Yaodong
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Li, Song
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Zhang, Yun
Ma, Zengling
Lu, Xiaoling
description The Arctic-derived fungus Eutypella sp. D-1 can produce numerous secondary metabolites, and some compounds exhibit excellent biological activity. Seven pimarane-type diterpenes, including three new compounds eutypellenone F (1), libertellenone Y (2), and libertellenone Z (3), and four known compounds (4–7), were isolated from fermentation broth of Eutypella sp. D-1 by the OSMAC strategy of adding ethanol as a promoter in the culture medium. Compound 2 has a rare tetrahydrofuran-fused pimarane diterpene skeleton. The anti-inflammatory activity of all compounds was evaluated. Compounds 3–6 showed a significant inhibitory effect on cell NO release at 10 μmol/L by in vitro experiments, of which 3–5 had inhibitory rates over 60% on nitric oxide (NO) release. Subsequently, the anti-inflammatory activity of 3–5 was evaluated based on a zebrafish model, and the results showed that 3 had a significant inhibitory effect on inflammatory cells migration at 40 μmol/L, while 4 and 5 had a significant inhibitory effect at 20 μmol/L. Moreover, compounds 3–5 have the same conjugated double bond structure, which may be an important group for these compounds to exert anti-inflammatory activity.
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subjects anti-inflammatory
Anti-inflammatory agents
Biological activity
Carbon
Cell culture
Cell migration
Culture media
diterpene
Diterpenes
Ethanol
Eutypella sp. D-1
Fermentation
Fungi
Inflammation
Metabolites
Migrations
Nitric oxide
Polar environments
Secondary metabolites
Spectrum analysis
Tetrahydrofuran
Zebrafish
zebrafish model
title Pimarane-Type Diterpenes with Anti-Inflammatory Activity from Arctic-Derived Fungus Eutypella sp. D-1
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