Sargachromenol Isolated from Sargassum horneri Inhibits Particulate Matter-Induced Inflammation in Macrophages through Toll-like Receptor-Mediated Cell Signaling Pathways

• Published in: Marine drugs 2021-12, Vol.20 (1), p.28
• Main Authors: Nagahawatta, D P, Kim, Hyun-Soo, Jee, Young-Heun, Jayawardena, Thilina U, Ahn, Ginnae, Namgung, Jin, Yeo, In-Kyu, Sanjeewa, K K Asanka, Jeon, You-Jin
• Format: Article
• Language: English
• Subjects: Algae; Animals; anti-inflammation; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anticoagulants; Aquatic Organisms; Arthritis; Benzopyrans - chemistry; Benzopyrans - pharmacology; Cell signaling; Coastal zone; Cosmeceuticals; Cytokines; Dust; ELISA; Enzyme-linked immunosorbent assay; Fisheries; Functional foods & nutraceuticals; Gene expression; Genetic engineering; Humans; Inflammation; Kinases; Lung diseases; Macrophages; Macrophages - metabolism; Mice; NF-κB protein; Nitric oxide; Particulate emissions; Particulate Matter; Phosphorylation; Plant Extracts - chemistry; Plant Extracts - pharmacology; Prostaglandin E2; Protein expression; Proteins; RAW 264.7 Cells - drug effects; Receptors; Respiratory diseases; Respiratory disorders; Sargachromenol; Sargassum; Sargassum horneri; Seaweeds; Secretion; Signal Transduction - drug effects; Suspended particulate matter; TLR; Toll-like receptors; Toll-Like Receptors - metabolism; Tumor necrosis factor-TNF; Western blotting
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20010028

is an invasive brown seaweed that grows along the shallow coastal areas of the Korean peninsula, which are potentially harmful to fisheries and natural habitats in the areas where it is accumulated. Therefore, the author attempted to evaluate the anti-inflammatory mechanism of Sargachromenol isolated from against particulate matter (PM)-stimulated RAW 264.7 macrophages. PM is a potent inducer of respiratory diseases such as lung dysfunctions and cancers. In the present study, the anti-inflammatory properties of Sargachromenol were validated using enzyme-linked immunosorbent assay (ELISA), Western blots, and RT-qPCR experiments. According to the results, Sargachromenol significantly downregulated the PM-induced proinflammatory cytokines, Prostaglandin E2 (PGE2), and Nitric Oxide (NO) secretion via blocking downstream activation of Toll-like receptor (TLR)-mediated nuclear factor kappa B (NF-κB) and MAPKs phosphorylation. Thus, Sargachromenol is a potential candidate for innovation in various fields including pharmaceuticals, cosmeceuticals, and functional food.