Septal stimulation attenuates hippocampal seizure with subregion specificity

• Published in: Epilepsia open 2024-08, Vol.9 (4), p.1445-1457
• Main Authors: Zhang, Qingyang, Wang, Yu, Wang, Fei, Jiang, Dongxiao, Song, Yingjie, Yang, Lin, Zhang, Mengdi, Wang, Yi, Ruan, Yeping, Fang, Jiajia, Fei, Fan
• Format: Article
• Language: English
• Subjects: Animals; cholinergic neuron; Convulsions & seizures; Deep Brain Stimulation; Diagonal Band of Broca; Disease Models, Animal; Drug resistance; Electrodes; Electroencephalography; Epilepsy; hippocampal rhythm; Hippocampus; Kainic Acid; Laboratory animals; Life sciences; Male; Mice; Mice, Inbred C57BL; Neurons; Original; seizure; Seizures - therapy; septum; Septum of Brain; Surgery
• ISSN: 2470-9239; 2470-9239
• DOI: 10.1002/epi4.12983

Objective Deep brain stimulation (DBS) is a promising approach for the treatment of epilepsy. However, the optimal target for DBS and underlying mechanisms are still not clear. Here, we compared the therapeutic effects of DBS on distinct septal subregions, aimed to find the precise targets of septal DBS and related mechanisms for the clinical treatment. Methods Assisted by behavioral test, electroencephalography (EEG) recording and analyzing, selectively neuronal manipulation and immunohistochemistry, we assessed the effects of DBS on the three septal subregions in kainic acid (KA)‐induced mouse seizure model. Results DBS in the medial septum (MS) not only delayed generalized seizure (GS) development, but reduced the severity; DBS in the vertical diagonal band of Broca (VDB) only reduced the severity of GS, while DBS in the horizontal diagonal band of Broca (HDB) subregion showed no anti‐seizure effect. Notably, DBS in the MS much more efficiently decreased abnormal activation of hippocampal neurons. EEG spectrum analysis indicated that DBS in the MS and VDB subregions mainly increased the basal hippocampal low‐frequency (delta and theta) rhythm. Furthermore, ablation of cholinergic neurons in the MS and VDB subregions blocked the anti‐seizure and EEG‐modulating effects of septal DBS, suggesting the seizure‐alleviating effect of DBS was dependent on local cholinergic neurons. Significance DBS in the MS and VDB, rather than HDB, attenuates hippocampal seizure by activation of cholinergic neurons‐augmented hippocampal delta/theta rhythm. This may be of great therapeutic significance for the clinical treatment of epilepsy with septal DBS. Plain Language Summary The optical target of deep brain stimulation in the septum is still not clear. This study demonstrated that stimulation in the medial septum and vertical diagonal band of Broca subregions, but not the horizontal diagonal band of Broca, could alleviate hippocampal seizure through cholinergic neurons‐augmented hippocampal delta/theta rhythm. This study may shed light on the importance of precise regulation of deep brain stimulation therapy in treating epileptic seizures.