Molecular Imaging of Steroid-Induced Osteonecrosis of the Femoral Head through iRGD-Targeted Microbubbles

Osteonecrosis of the femoral head (ONFH) is a disease that is commonly seen in the clinic, but its detection rate remains limited, especially at the early stage. We developed an ultrasound molecular imaging (UMI) approach for early diagnosis of ONFH by detecting the expression of integrin αvβ3 durin...

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Bibliographic Details
Published in:Pharmaceutics 2022-09, Vol.14 (9), p.1898
Main Authors: Zhao, Ping, Zhao, Shuai, Zhang, Jiaqi, Lai, Manlin, Sun, Litao, Yan, Fei
Format: Article
Language:English
Subjects:
Blood vessels
Bones
Complications and side effects
Extracellular matrix
Hip
integrin αvβ3
iRGD peptides
Medical examination
Medical imaging
Methods
Microbubbles
Molecular diagnostic techniques
Necrosis
osteonecrosis of the femoral head
Particle size
Peptides
Polyvinyl alcohol
Quality of life
Radiation
Steroids
Steroids (Drugs)
Ultrasonic imaging
Ultrasound imaging
ultrasound molecular imaging
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Summary:Osteonecrosis of the femoral head (ONFH) is a disease that is commonly seen in the clinic, but its detection rate remains limited, especially at the early stage. We developed an ultrasound molecular imaging (UMI) approach for early diagnosis of ONFH by detecting the expression of integrin αvβ3 during the pathological changes in steroid-induced osteonecrosis of the femoral head (SIONFH) in rat models. The integrin αvβ3-targeted PLGA or lipid microbubbles modified with iRGD peptides were fabricated and characterized. Their adhesion efficiency to mouse brain microvascular endothelial cells in vitro was examined, and the better LIPOiRGD was used for further in vivo molecular imaging of SIONFH rats at 1, 3 and 5 weeks; revealing significantly higher UMI signals could be observed in the 3-week and 5-week SIONFH rats but not in the 1-week SIONFH rats in comparison with the non-targeted microbubbles (32.75 ± 0.95 vs. 0.17 ± 0.09 for 5 weeks, p < 0.05; 5.60 ± 1.31 dB vs. 0.94 ± 0.81 dB for 3 weeks, p < 0.01; 1.13 ± 0.13 dB vs. 0.73 ± 0.31 dB for 1 week, p > 0.05). These results were consistent with magnetic resonance imaging data and confirmed by immunofluorescence staining experiments. In conclusion, our study provides an alternative UMI approach to the early evaluation of ONFH.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14091898