Circulating Placental Vesicles Carry HLA-DR in Pre-Eclampsia: A New Potential Marker of the Syndrome

Pre-eclampsia (PE) is a common disorder of pregnancy that usually presents with hypertension and proteinuria. The clinical presentation arises from soluble factors released into the maternal circulation from the placenta owing to the stress of syncytiotrophoblast, consequence of defective placentati...

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Bibliographic Details
Published in:Frontiers in immunology 2021-09, Vol.12, p.717879-717879
Main Authors: Tersigni, Chiara, Lucchetti, Donatella, Franco, Rita, Colella, Filomena, Neri, Caterina, Crispino, Laura, Sgambato, Alessandro, Lanzone, Antonio, Scambia, Giovanni, Vatish, Manu, Di Simone, Nicoletta
Format: Article
Language:English
Subjects:
Adult
Biomarkers - blood
Disease Susceptibility
Extracellular Vesicles - metabolism
Female
Flow Cytometry
HLA-DR Antigens - immunology
HLA-DR Antigens - metabolism
human leukocyte antigen
Humans
Immunology
placenta
Placenta - immunology
Placenta - metabolism
pre-eclampsia
Pre-Eclampsia - blood
Pre-Eclampsia - etiology
Pre-Eclampsia - metabolism
Pregnancy
Prognosis
syncytiotrophoblast extracellular vesicles
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Summary:Pre-eclampsia (PE) is a common disorder of pregnancy that usually presents with hypertension and proteinuria. The clinical presentation arises from soluble factors released into the maternal circulation from the placenta owing to the stress of syncytiotrophoblast, consequence of defective placentation occurring in the first half of pregnancy. Reduced tolerance of the semiallogeneic fetus by the maternal immune system has been proposed as first trigger leading to poor placentation. We previously observed aberrant expression of human leukocyte antigen (HLA)-DR molecules in the syncytiotrophoblast of a subset of women with PE. Aim of this study was to investigate abnormal expression of circulating HLA-DR in syncytiotrophoblast-derived extracellular vesicles (STBEVs) in women with PE compared to normal pregnant women. peripheral venous blood was collected from 22 women with PE and 22 normal pregnant women. Circulating STBEVs were collected by ultra-centrifugation (120000 g) and analyzed for the expression of HLA-DR and placental alkaline phosphatase (PLAP), a specific marker of the placenta, by Western blot analysis and flow cytometry. circulating STBEVs positive for HLA-DR were observed in 64% of PE women while no HLA-DR positivity was detected in any of the controls (P
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2021.717879