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Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. In this research, we g...
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| Published in: | Biological research 2017-04, Vol.50 (1), p.14-14, Article 14 |
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| creator | Mata-Miranda, Monica Maribel Vazquez-Zapien, Gustavo Jesus Rojas-Lopez, Marlon Sanchez-Monroy, Virginia Perez-Ishiwara, David Guillermo Delgado-Macuil, Raul Jacobo |
| description | Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.
In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.
Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. |
| doi_str_mv | 10.1186/s40659-017-0119-6 |
| format | article |
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In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.
Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.</description><identifier>ISSN: 0717-6287</identifier><identifier>ISSN: 0716-9760</identifier><identifier>EISSN: 0717-6287</identifier><identifier>DOI: 10.1186/s40659-017-0119-6</identifier><identifier>PMID: 28376862</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Animals ; Cell Differentiation - physiology ; Cells, Cultured ; Diabetes ; Dialysis ; Differentiated kidney cells ; Embryogenesis ; Embryos ; Fluorescent Antibody Technique ; Fourier analysis ; Fourier transform infrared ; Gene Expression ; Immunohistochemistry ; Infrared spectroscopy ; Kidney - cytology ; Mice ; Morbidity ; Morphology ; Mortality ; Pluripotency ; Pluripotent stem cells ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - physiology ; Principal Component Analysis ; Principal components analysis ; Proteins ; Real-Time Polymerase Chain Reaction ; Regenerative medicine ; Spectroscopy, Fourier Transform Infrared - methods ; Spectrum analysis ; Stem cell transplantation ; Stem cells ; Transplants & implants ; Vibrational spectroscopy</subject><ispartof>Biological research, 2017-04, Vol.50 (1), p.14-14, Article 14</ispartof><rights>Copyright BioMed Central 2017</rights><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-fdacc3841bb41e0af65be4322000ca3a752d255c2b5be470ab69a413424d68bd3</citedby><cites>FETCH-LOGICAL-c493t-fdacc3841bb41e0af65be4322000ca3a752d255c2b5be470ab69a413424d68bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1894419173?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,25732,27903,27904,36991,36992,44569</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28376862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mata-Miranda, Monica Maribel</creatorcontrib><creatorcontrib>Vazquez-Zapien, Gustavo Jesus</creatorcontrib><creatorcontrib>Rojas-Lopez, Marlon</creatorcontrib><creatorcontrib>Sanchez-Monroy, Virginia</creatorcontrib><creatorcontrib>Perez-Ishiwara, David Guillermo</creatorcontrib><creatorcontrib>Delgado-Macuil, Raul Jacobo</creatorcontrib><title>Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells</title><title>Biological research</title><addtitle>Biol Res</addtitle><description>Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.
In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.
Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.</description><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Diabetes</subject><subject>Dialysis</subject><subject>Differentiated kidney cells</subject><subject>Embryogenesis</subject><subject>Embryos</subject><subject>Fluorescent Antibody Technique</subject><subject>Fourier analysis</subject><subject>Fourier transform infrared</subject><subject>Gene Expression</subject><subject>Immunohistochemistry</subject><subject>Infrared spectroscopy</subject><subject>Kidney - cytology</subject><subject>Mice</subject><subject>Morbidity</subject><subject>Morphology</subject><subject>Mortality</subject><subject>Pluripotency</subject><subject>Pluripotent stem cells</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - physiology</subject><subject>Principal Component Analysis</subject><subject>Principal components analysis</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regenerative medicine</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Spectrum analysis</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Transplants & implants</subject><subject>Vibrational spectroscopy</subject><issn>0717-6287</issn><issn>0716-9760</issn><issn>0717-6287</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdUl1rFDEUHUSxtfoDfJGALz50Nd_JvAilWF2oCFKfw50ks5s1MxmTGWGf_OvNurW0PoQk555zyM09TfOa4PeEaPmhcCxFu8JE1UXalXzSnGJVb5Jq9fTB-aR5UcoOYyowlc-bE6qZklrS0-bP15SnbYppEyzEczSk6O0SISMYHbq6WX9HZfJ2zqnYNAVbYYj7EgpySw7jBs1bj1zoe5_9OAeYQxpR6tHP4Ea_R9bHWFCf04CmWAVTmisNldkPx9rL5lkPsfhXd_tZ8-Pq083ll9X1t8_ry4vrleUtm1e9A2uZ5qTrOPEYeik6zxmlGGMLDJSgjgphaXfAFYZOtsAJ45Q7qTvHzpr10dcl2JkphwHy3iQI5i-Q8sZAnoON3lCGu97LjigruBcCgHgLUivvhGg1rV4fj17T0g3e2dpRhvjI9HFlDFuzSb-NYKqVGleDd3cGOf1afJnNEMrhO2D0aSmGaM255IKqSn37H3WXllxncGC1nJOWKFZZ5MiydU4l-_7-MQSbQ1TMMSqmRsUcomJk1bx52MW94l822C1kvrz2</recordid><startdate>20170404</startdate><enddate>20170404</enddate><creator>Mata-Miranda, Monica Maribel</creator><creator>Vazquez-Zapien, Gustavo Jesus</creator><creator>Rojas-Lopez, Marlon</creator><creator>Sanchez-Monroy, Virginia</creator><creator>Perez-Ishiwara, David Guillermo</creator><creator>Delgado-Macuil, Raul Jacobo</creator><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170404</creationdate><title>Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells</title><author>Mata-Miranda, Monica Maribel ; Vazquez-Zapien, Gustavo Jesus ; Rojas-Lopez, Marlon ; Sanchez-Monroy, Virginia ; Perez-Ishiwara, David Guillermo ; Delgado-Macuil, Raul Jacobo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-fdacc3841bb41e0af65be4322000ca3a752d255c2b5be470ab69a413424d68bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Diabetes</topic><topic>Dialysis</topic><topic>Differentiated kidney cells</topic><topic>Embryogenesis</topic><topic>Embryos</topic><topic>Fluorescent Antibody Technique</topic><topic>Fourier analysis</topic><topic>Fourier transform infrared</topic><topic>Gene Expression</topic><topic>Immunohistochemistry</topic><topic>Infrared spectroscopy</topic><topic>Kidney - cytology</topic><topic>Mice</topic><topic>Morbidity</topic><topic>Morphology</topic><topic>Mortality</topic><topic>Pluripotency</topic><topic>Pluripotent stem cells</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - physiology</topic><topic>Principal Component Analysis</topic><topic>Principal components analysis</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regenerative medicine</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>Spectrum analysis</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Transplants & implants</topic><topic>Vibrational spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mata-Miranda, Monica Maribel</creatorcontrib><creatorcontrib>Vazquez-Zapien, Gustavo Jesus</creatorcontrib><creatorcontrib>Rojas-Lopez, Marlon</creatorcontrib><creatorcontrib>Sanchez-Monroy, Virginia</creatorcontrib><creatorcontrib>Perez-Ishiwara, David Guillermo</creatorcontrib><creatorcontrib>Delgado-Macuil, Raul Jacobo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mata-Miranda, Monica Maribel</au><au>Vazquez-Zapien, Gustavo Jesus</au><au>Rojas-Lopez, Marlon</au><au>Sanchez-Monroy, Virginia</au><au>Perez-Ishiwara, David Guillermo</au><au>Delgado-Macuil, Raul Jacobo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells</atitle><jtitle>Biological research</jtitle><addtitle>Biol Res</addtitle><date>2017-04-04</date><risdate>2017</risdate><volume>50</volume><issue>1</issue><spage>14</spage><epage>14</epage><pages>14-14</pages><artnum>14</artnum><issn>0717-6287</issn><issn>0716-9760</issn><eissn>0717-6287</eissn><abstract>Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.
In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.
Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>28376862</pmid><doi>10.1186/s40659-017-0119-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | SpringerLink Contemporary; Publicly Available Content Database; SciELO |
| subjects | Animals Cell Differentiation - physiology Cells, Cultured Diabetes Dialysis Differentiated kidney cells Embryogenesis Embryos Fluorescent Antibody Technique Fourier analysis Fourier transform infrared Gene Expression Immunohistochemistry Infrared spectroscopy Kidney - cytology Mice Morbidity Morphology Mortality Pluripotency Pluripotent stem cells Pluripotent Stem Cells - cytology Pluripotent Stem Cells - physiology Principal Component Analysis Principal components analysis Proteins Real-Time Polymerase Chain Reaction Regenerative medicine Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Stem cell transplantation Stem cells Transplants & implants Vibrational spectroscopy |
| title | Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells |
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