Systematic Analysis of the bZIP Family in Tobacco and Functional Characterization of NtbZIP62 Involvement in Salt Stress

The basic leucine zipper (bZIP) transcription factors play important regulatory roles, influencing plant growth and responses to environmental stresses. In the present study, 132 bZIP genes identified in the tobacco genome were classified into 11 groups with Arabidopsis and tomato bZIP members, base...

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Published in:Agronomy (Basel) 2021-01, Vol.11 (1), p.148
Main Authors: Li, Zhiyuan, Chao, Jiangtao, Li, Xiaoxu, Li, Gongbo, Song, Dean, Guo, Yongfeng, Wu, Xinru, Liu, Guanshan
Format: Article
Language:English
Subjects:
Abiotic stress
Biosynthesis
biotic stress
bZIP
Cloning
Environmental stress
Gene expression
gene family
Genes
Genomes
Homology
Leucine
Leucine zipper proteins
Localization
Pathogens
Phylogeny
Plant growth
Proteins
Reproduction (copying)
Salinity tolerance
Stresses
Tobacco
Tomatoes
Transcription factors
Transgenic plants
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Summary:The basic leucine zipper (bZIP) transcription factors play important regulatory roles, influencing plant growth and responses to environmental stresses. In the present study, 132 bZIP genes identified in the tobacco genome were classified into 11 groups with Arabidopsis and tomato bZIP members, based on the results of a phylogenetic analysis. An examination of gene structures and conserved motifs revealed relatively conserved exon/intron structures and motif organization within each group. The results of an investigation of whole-genome duplication events indicated that segmental duplications were crucial for the expansion of the bZIP gene family in tobacco. Expression profiles confirmed that the NtbZIP genes are differentially expressed in various tissues, and several genes are responsive to diverse stresses. Notably, NtbZIP62, which was identified as an AtbZIP37/ABF3 homolog, was highly expressed in response to salinity. Subcellular localization analyses proved that NtbZIP62 is a nuclear protein. Furthermore, the overexpression of NtbZIP62 in tobacco significantly enhanced the salt stress tolerance of the transgenic plants. The results of this study may be relevant for future functional analyses of the bZIP genes in tobacco.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy11010148