Extraction of Bioactive Compounds from C. vulgaris Biomass Using Deep Eutectic Solvents

microalgae biomass was employed for the extraction of valuable bioactive compounds with deep eutectic-based solvents (DESs). Particularly, the Choline Chloride (ChCl) based DESs, ChCl:1,2 butanediol (1:4), ChCl:ethylene glycol (1:2), and ChCl:glycerol (1:2) mixed with water at 70/30 / ratio were use...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-01, Vol.28 (1), p.415
Main Authors: Dardavila, Maria Myrto, Pappou, Sofia, Savvidou, Maria G, Louli, Vasiliki, Katapodis, Petros, Stamatis, Haralambos, Magoulas, Kostis, Voutsas, Epaminondas
Format: Article
Language:English
Subjects:
Algae
antioxidant activity
Antioxidants
Bioactive compounds
Biological activity
Biomass
Butanediol
C. vulgaris
carotenoid content
Carotenoids
Chlorella
Choline
DES
Design of experiments
Design optimization
Efficiency
Eutectic temperature
Experimental design
Glycerol
Hydrogen bonds
Phenolic compounds
phenolic content
Phenols
Process parameters
Response surface methodology
RSM
Solvents
Statistical analysis
Viscosity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:microalgae biomass was employed for the extraction of valuable bioactive compounds with deep eutectic-based solvents (DESs). Particularly, the Choline Chloride (ChCl) based DESs, ChCl:1,2 butanediol (1:4), ChCl:ethylene glycol (1:2), and ChCl:glycerol (1:2) mixed with water at 70/30 / ratio were used for that purpose. The extracts' total carotenoid (TCC) and phenolic contents (TPC), as well as their antioxidant activity (IC50), were determined within the process of identification of the most efficient solvent. This screening procedure revealed ChCl:1,2 butanediol (1:4)/H O 70/30 / as the most compelling solvent; thus, it was employed thereafter for the extraction process optimization. Three extraction parameters, i.e., solvent-to-biomass ratio, temperature, and time were studied regarding their impact on the extract's TCC, TPC, and IC50. For the experimental design and process optimization, the statistical tool Response Surface Methodology was used. The resulting models' predictive capacity was confirmed experimentally by carrying out two additional extractions under conditions different from the experimental design.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28010415