A SIMPLE AND SENSITIVE LC-MS/MS METHOD FOR THE DETERMINATION OF S-PHENYLMERCAPTURIC ACID IN HUMAN URINE

Benzene is an important environmental and occupational pollutant, and recognized as human carcinogen. S-phenylmercapturic acid (SPMA) is a highly specific biomarker of exposure to benzene, applied in occupational toxicology to assess low levels of benzene exposure. The objective of this study was to...

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Bibliographic Details
Published in:Química Nova 2021-01, Vol.44 (3), p.334-340
Main Authors: Gomes, Andressa, Barbosa, Eduardo, Santos, Ana, Lizot, Lilian, Sauer, Elisa, Garcia, Solange, Linden, Rafael, Antunes, Marina, Charao, Mariele
Format: Article
Language:English
Subjects:
benzene
CHEMISTRY, MULTIDISCIPLINARY
LC-MS/MS
occupational toxicology
S-phenylmercapturic acid
validation
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Summary:Benzene is an important environmental and occupational pollutant, and recognized as human carcinogen. S-phenylmercapturic acid (SPMA) is a highly specific biomarker of exposure to benzene, applied in occupational toxicology to assess low levels of benzene exposure. The objective of this study was to develop and validate a one-step liquid extraction bioanalytical method for the quantification of SPMA in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was applied to the evaluation of benzene exposure in an occupational setting. The assay was linear from 0.5 to 500 ng mL-1 (r>0.99), accurate (91.4-105.2%) and precise, with CV% between 4.73 and 9.96%. SPMA was stable in urine for 90 days at -20 °C. Airborne benzene concentrations, urinary levels of SPMA and t,t-MA were significantly higher in gas station workers (n=30) in comparison to outdoor workers (n=14) and individuals non-occupationally exposed to benzene (n=34). Benzene airborne levels had higher correlation with urinary SPMA (r=0.532, p
ISSN:0100-4042
1678-7064
1678-7064
DOI:10.21577/0100-4042.20170651