‘Lactobacillus sp. strain TERI-D3’, as microbial cell factory for fermentative production of lactic acid

• Published in: Current research in green and sustainable chemistry 2021, Vol.4, p.100059, Article 100059
• Main Authors: Verma, Dipti, Subudhi, Sanjukta
• Format: Article
• Language: English
• Subjects: Anaerobic fermentation; Disaccharides; Lactic acid; Lactobacillus sp; Monosaccharides; Rice straw biomass; Strain TERI-D3
• ISSN: 2666-0865; 2666-0865
• DOI: 10.1016/j.crgsc.2021.100059

This study reports for lactic acid production from different carbohydrates; monosaccharide (glucose, galactose, lactose) & disaccharides (sucrose) and from lignocellulose biomass (rice straw) by a novel strain ‘Lactobacillus sp. strain TERI-D3’. ‘TERI-D3’ strain produced 19.9, 19.4, 18.1 and 15.8 ​g/L of lactic acid from glucose, sucrose, lactose and galactose, respectively. Maximum lactic acid yield efficiency (0.97 ​g/g) was observed with glucose (>95% of the theoretical maximum yield). Lactic acid titer from glucose was 0.41 ​g/L. The lactic acid titer and yield from rice straw biomass sugar was; 11.58 ​g/L and 0.73 ​g/g (>80% of the theoretical maximum yield), respectively. The novelty of this study is that the ‘TERI-D3’ strain is a promising microbe for green lactic acid as it has potential to valorize cheese industry waste, algae biomass as well as to utilize inexpensive next generation lignocellulose biomass that are abundant and do not compete with food chain supply. This approach of recycling and or reuse of organic waste holds importance in the circular economy frame. •A novel strain identified as Lactobacillus sp. strain TERI-D3 isolated from spent dairy water.•TERI-D3 strain utilized mono- and Di-saccharides to produce lactic acid with >95% yield efficiency.•This strain also valorised rice straw biomass sugar to produce lactic acid with 96% yield efficiency.•Lactic ​acid productivity of this strain from glucose was 0.41 g/L/h.