Anti-Adipogenic Activity of High-Phenolic Sorghum Brans in Pre-Adipocytes

• Published in: Nutrients 2022-04, Vol.14 (7), p.1493
• Main Authors: Lee, Hee-Seop, Santana, Ádina L, Peterson, Jaymi, Yucel, Umut, Perumal, Ramasamy, De Leon, Joaquin, Lee, Seong-Ho, Smolensky, Dmitriy
• Format: Article
• Language: English
• Subjects: 3T3-L1; 3T3-L1 Cells; Acids; Adipocytes; Adipocytes - metabolism; Adipogenesis; Animals; Body mass index; Cardiovascular diseases; Cell differentiation; Cereal crops; Cytotoxicity; Diabetes; Diabetes mellitus; Diet; Edible Grain; Ethanol; Genotype & phenotype; Genotypes; Grain; Health problems; high-phenolic sorghum bran; Insulin; Lipids; lipogenesis; MAP kinase; Mice; Obesity; Obesity - metabolism; Phenolic compounds; Phenols; Phenols - metabolism; Phenols - pharmacology; Plant Extracts - metabolism; Plant Extracts - pharmacology; polyphenols; Polyphenols - metabolism; Polyphenols - pharmacology; Preadipocytes; Public health; Reactive oxygen species; Reagents; Sorghum; Sumac; Weight control
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14071493

Obesity is one of the leading public health problems that can result in life-threatening metabolic and chronic diseases such as cardiovascular diseases, diabetes, and cancer. Sorghum ( (L.) Moench) is the fifth most important cereal crop in the world and certain genotypes of sorghum have high polyphenol content. PI570481, SC84, and commercially available sumac sorghum are high-polyphenol genotypes that have demonstrated strong anti-cancer activities in previous studies. The objective of this study was to explore a potential anti-obesity use of extracts from sorghum bran in the differentiation of 3T3-L1 preadipocytes and to investigate cellular and molecular responses in differentiated adipocytes to elucidate related mechanisms. None of the four different sorghum bran extracts (PI570481, SC84, Sumac, and white sorghum as a low-polyphenol control) caused cytotoxicity in undifferentiated and differentiated 3T3-L1 cells at doses used in this study. Sorghum bran extracts (PI570481, SC84, and Sumac) reduced intracellular lipid accumulation and expression of adipogenic and lipogenic proteins in a dose-dependent manner in differentiated 3T3-L1 cells. The same polyphenol containing sorghum bran extracts also repressed production of reactive oxygen species (ROS) and MAPK signaling pathways and repressed insulin signaling and glucose uptake in differentiated 3T3-L1 cells. These data propose a potential use of high-phenolic sorghum bran for the prevention of obesity.