Application of Reverse Genetics in Functional Genomics of Potyvirus

Numerous potyvirus studies, including virus biology, transmission, viral protein function, as well as virus-host interaction, have greatly benefited from the utilization of reverse genetic techniques. Reverse genetics of RNA viruses refers to the manipulation of viral genomes, transfection of the mo...

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Bibliographic Details
Published in:Viruses 2020-07, Vol.12 (8), p.803
Main Authors: Kannan, Maathavi, Zainal, Zamri, Ismail, Ismanizan, Baharum, Syarul Nataqain, Bunawan, Hamidun
Format: Article
Language:English
Subjects:
Genome, Viral
Genomics - methods
Host Microbial Interactions
Plant Diseases - virology
potyvirus
Potyvirus - genetics
reverse genetics
Reverse Genetics - methods
Review
RNA, Viral - genetics
transmission
viral protein
Viral Proteins - genetics
virus biology
virus–host interaction
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Summary:Numerous potyvirus studies, including virus biology, transmission, viral protein function, as well as virus-host interaction, have greatly benefited from the utilization of reverse genetic techniques. Reverse genetics of RNA viruses refers to the manipulation of viral genomes, transfection of the modified cDNAs into cells, and the production of live infectious progenies, either wild-type or mutated. Reverse genetic technology provides an opportunity of developing potyviruses into vectors for improving agronomic traits in plants, as a reporter system for tracking virus infection in hosts or a production system for target proteins. Therefore, this review provides an overview on the breakthroughs achieved in potyvirus research through the implementation of reverse genetic systems.
ISSN:1999-4915
1999-4915
DOI:10.3390/v12080803