Multi-Color Enhanced Fluorescence Imaging of a Breast Cancer Cell with A Hole-Arrayed Plasmonic Chip

Breast cancer cells of MDA-MB-231 express various types of membrane proteins in the cell membrane. In this study, two types of membrane proteins in MDA-MB-231 cells were observed using a plasmonic chip with an epifluorescence microscope. The targeted membrane proteins were epithelial cell adhesion m...

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Bibliographic Details
Published in:Micromachines (Basel) 2020-06, Vol.11 (6), p.604
Main Authors: Yoshida, Makiko, Chida, Hinako, Kimura, Fukiko, Yamamura, Shohei, Tawa, Keiko
Format: Article
Language:English
Subjects:
Adsorption
Antibodies
Breast cancer
breast cancer cells
Cell adhesion
Cell adhesion & migration
Cell membranes
Color
Electric fields
Fluorescence
fluorescence microscopy
Glass substrates
Growth factors
hole-array
Light
Microscopy
multi-color imaging
Plasmonics
Proteins
Resonance
Silver
surface plasmon
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Summary:Breast cancer cells of MDA-MB-231 express various types of membrane proteins in the cell membrane. In this study, two types of membrane proteins in MDA-MB-231 cells were observed using a plasmonic chip with an epifluorescence microscope. The targeted membrane proteins were epithelial cell adhesion molecules (EpCAMs) and epidermal growth factor receptor (EGFR), and Alexa 488-EGFR antibody and allophycocyanin (APC)-labeled EpCAM antibody were applied to the fluorescent detection. The plasmonic chip used in this study is composed of a two-dimensional hole-array structure, which is expected to enhance the fluorescence at different resonance wavelengths due to two kinds of grating pitches in a square side and a diagonal direction. As a result of multi-color imaging, the enhancement factor of Alexa 488-EGFR and APC-EpCAM was 13 ± 2 and 12 ± 2 times greater on the plasmonic chip, respectively. The excited wavelength or emission wavelength of each fluorescent agent is due to consistency with plasmon resonance wavelength in the hole-arrayed chip. The multi-color fluorescence images of breast cancer cells were improved by the hole-arrayed plasmonic chip.
ISSN:2072-666X
2072-666X
DOI:10.3390/mi11060604