Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy: In Vitro Study in Multi-Species Oral Biofilm

The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not...

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Bibliographic Details
Published in:Pathogens (Basel) 2024-04, Vol.13 (4), p.342
Main Authors: Bueno-Silva, Bruno, Parma-Garcia, Javier, Frigo, Lucio, Suárez, Lina J, Macedo, Tatiane Tiemi, Uyeda, Fábio Hideaki, Melo, Marcelo Augusto Ruiz da Cunha, Sacco, Roberto, Mourão, Carlos Fernando, Feres, Magda, Shibli, Jamil Awad, Figueiredo, Luciene Cristina
Format: Article
Language:English
Subjects:
Anti-infective agents
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Bacteria
Biofilms
Campylobacter
Cancer
Communicable diseases
Complications and side effects
Composition
Ethylenediaminetetraacetic acid
Health aspects
Infectious diseases
laser
Light-emitting diodes
Metabolism
Methylene blue
Microbial mats
Microorganisms
Patient outcomes
peri-implantitis
periodontal diseases
Photochemotherapy
Photodynamic therapy
Sheep
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Summary:The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not been tested in polymicrobial biofilm models. This study evaluated the effect of photodynamic therapy in vitro with methylene blue (MB) 0.01% and red LEDs (λ = 660 nm, power density ≈ 330 mW/cm , 2 mm distance from culture) on the metabolic activity and composition of a multispecies subgingival biofilm. Test Groups LED and MB + LED showed a more significant reduction in metabolic activity than the non-LED application group (~50 and 55%, respectively). Groups LED and MB equally affected (more than 80%) the total bacterial count in biofilms. No differences were noted in the bacterial biofilm composition between the groups. In vitro LED alone or the MB + LED combination reduced the metabolic activity of bacteria in polymicrobial biofilms and the total subgingival biofilm count.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens13040342