Characterization and functional analyses of wheat TaPR1 genes in response to stripe rust fungal infection

The pathogenesis-related protein-1 (PR1) gene is important for plants to respond to various biotic and abiotic stresses. Unlike those in model plants, PR1 genes in wheat have not been systematically studied. Herein, we identified 86 potential TaPR1 wheat genes using bioinformatics tools and RNA sequ...

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Bibliographic Details
Published in:Scientific reports 2023-02, Vol.13 (1), p.3362-3362, Article 3362
Main Authors: Liu, Rong, Lu, Jing, Xing, Jiayi, Xue, Lv, Wu, Yu, Zhang, Lei
Format: Article
Language:English
Subjects:
631/449/2491
631/449/711
Abiotic stress
Apoptosis
Barley
Basidiomycota
Bioinformatics
Chromosomes
Computational Biology
Connective Tissue Diseases
Disease
Disease resistance
Eczema
Flowers & plants
Fungal infections
Gene silencing
Genes
Genomes
Humanities and Social Sciences
MAP kinase
multidisciplinary
Mycoses
Pathogenesis
Pathogens
Phenylalanine
Proteins
Salicylic acid
Science
Science (multidisciplinary)
Signal Transduction
Skin Diseases, Bacterial
Stripe rust
Triticum - genetics
Wheat
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Summary:The pathogenesis-related protein-1 (PR1) gene is important for plants to respond to various biotic and abiotic stresses. Unlike those in model plants, PR1 genes in wheat have not been systematically studied. Herein, we identified 86 potential TaPR1 wheat genes using bioinformatics tools and RNA sequencing. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the TaPR1 genes were involved in the salicylic acid signalling pathway, MAPK signalling pathway, and phenylalanine metabolism in response to Pst-CYR34 infection. Ten of the TaPR1 genes were structurally characterized and validated by RT‒PCR. One particular gene, TaPR1-7, was found to be associated with resistance to Puccinia striiformis f. sp. tritici (Pst) in a biparental wheat population. Virus-induced gene silencing showed that TaPR1-7 is important for Pst resistance in wheat. This study provides the first comprehensive study on wheat PR1 genes, improving our overall understanding of these genes in plant defenses, particularly against stripe rust.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-30456-8