Citrulluside T, Isolated from the Citrullus lanatus Stem, Inhibits Melanogenesis in α-MSH-Induced Mouse B16F10 Cells

With the increasing number of cosmetic consumers emphasizing value consumption and sustainability, upcycling has gained attention as a solution to agricultural by-products, which are the main culprits of environmental problems. In this study, we isolated citrulluside T with whitening activity from d...

Full description

Saved in:
Bibliographic Details
Published in:Cosmetics (Basel) 2023-08, Vol.10 (4), p.108
Main Authors: Kim, Hyeon-Mi, Moon, Mi-Yeon, Hyun, Chang-Gu
Format: Article
Language:English
Subjects:
Acids
By products
Chromatography
Citrullus lanatus
citrulluside T
Citrus fruits
Coffee
Cosmetics
Cyclic AMP response element-binding protein
Cytotoxicity
Enzymes
Hyperpigmentation
Investigations
Irritation
mechanisms
Melanin
melanogenesis
Melanoma
Microphthalmia-associated transcription factor
NMR
Nuclear magnetic resonance
Phytochemicals
Protein biosynthesis
Protein kinase A
skin irritation
Skin tests
Transcription factors
upcycling
β-Catenin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:With the increasing number of cosmetic consumers emphasizing value consumption and sustainability, upcycling has gained attention as a solution to agricultural by-products, which are the main culprits of environmental problems. In this study, we isolated citrulluside T with whitening activity from discarded Citrullus lanatus stems and investigated the anti-melanogenic effect of citrulluside T and the underlying mechanisms. We found that citrulluside T did not exhibit cytotoxicity up to a concentration of 90 μM and significantly reduced the melanin content and intracellular tyrosinase activity in B16F10 cells. In addition, citrulluside T inhibited the expression of melanogenic enzymes such as tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2, as well as melanin synthesis via cAMP-dependent protein kinase (PKA)/cAMP response element-binding protein (CREB)-mediated downregulation of microphthalmia-associated transcription factor (MITF), a key transcription factor in melanogenesis. Furthermore, we found that citrulluside T exerted its anti-melanogenic effect by downregulating the β-catenin protein and upregulating phosphorylated β-catenin. Finally, we confirmed that citrulluside T was safe for skin through skin irritation tests on 33 subjects, suggesting its applicability as a protective agent against hyperpigmentation for topical applications such as cosmetics and ointments.
ISSN:2079-9284
2079-9284
DOI:10.3390/cosmetics10040108