Methylene blue promotes quiescence of rat neural progenitor cells

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited prolif...

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Bibliographic Details
Published in:Frontiers in cellular neuroscience 2014-10, Vol.8, p.315-315
Main Authors: Xie, Luokun, Choudhury, Gourav R, Wang, Jixian, Park, Yong, Liu, Ran, Yuan, Fang, Zhang, Chun-Li, Yorio, Thomas, Jin, Kunlin, Yang, Shao-Hua
Format: Article
Language:English
Subjects:
Aging
Autophagy
Cell cycle
Cell differentiation
Cell division
Cell growth
Cell proliferation
Cyclins
Epidermal growth factor
Fibroblasts
Homeostasis
Kinases
Metabolism
Methylene Blue
Microscopy
neural progenitor cell
Neural stem cells
Neurodegenerative diseases
Neurogenesis
Neuroscience
Progenitor cells
proliferation
quiescence
Rodents
Senescence
Stem cells
Subventricular zone
TOR protein
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Summary:Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.
ISSN:1662-5102
1662-5102
DOI:10.3389/fncel.2014.00315