RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species

RNA hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model species. We describe best practices in pupal staging, tissu...

Full description

Saved in:
Bibliographic Details
Published in:Methods and protocols 2021-03, Vol.4 (1), p.20
Main Authors: Shittu, Mujeeb, Steenwinkel, Tessa, Dion, William, Ostlund, Nathan, Raja, Komal, Werner, Thomas
Format: Article
Language:English
Subjects:
Abdomen
Cloning
Design
Developmental biology
Dissection
Drosophila
Evolutionary genetics
Gene expression
Hybridization
in situ hybridization
Insects
Protocol
RNA polymerase
Species
Wings
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RNA hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model species.
ISSN:2409-9279
2409-9279
DOI:10.3390/mps4010020