Classical and Modern Models for Biofilm Studies: A Comprehensive Review

Biofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistan...

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Bibliographic Details
Published in:Antibiotics (Basel) 2024-12, Vol.13 (12), p.1228
Main Authors: Yang, Zhihe, Khan, Sadaf Aiman, Walsh, Laurence J, Ziora, Zyta M, Seneviratne, Chaminda Jayampath
Format: Article
Language:English
Subjects:
3D printing
Analysis
Antibiotics
Antimicrobial agents
Bacteria
Bioengineering
Biofilms
Biomass
Dental implants
Drug resistance
Extracellular matrix
Gram-positive bacteria
Gum disease
Influence
Laboratories
laboratory models
Medical equipment
micro-environments
Microbial activity
Microorganisms
Nutrients
Polymers
Polysaccharides
Review
Temperature
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Summary:Biofilms are structured microbial communities that adhere to various abiotic and biotic surfaces, where organisms are encased in an exo-polysaccharide matrix. Organisms within biofilms use various mechanisms that help them resist external challenges, such as antibiotics, rendering them more resistant to drugs. Therefore, researchers have attempted to develop suitable laboratory models to study the physical features of biofilms, their resistance mechanisms against antimicrobial agents, and their gene and protein expression profiles. However, current laboratory models suffer from various limitations. In this comprehensive review, we have summarized the various designs that have been used for laboratory biofilm models, presenting their strengths and limitations. Additionally, we have provided insight into improving these models to more closely simulate real-life scenarios, using newly developed techniques in additive manufacturing, synthetic biology, and bioengineering.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics13121228