Repair abilities of mouse autologous adipose-derived stem cells and ShakeGel™3D complex local injection with intrauterine adhesion by BMP7-Smad5 signaling pathway activation

• Published in: Stem cell research & therapy 2021-03, Vol.12 (1), p.191-191, Article 191
• Main Authors: Zhao, Yun-Xia, Chen, Shao-Rong, Huang, Qiao-Yi, Chen, Wei-Can, Xia, Tian, Shi, Yan-Chuan, Gao, Hong-Zhi, Shi, Qi-Yang, Lin, Shu
• Format: Article
• Language: English
• Subjects: Adhesion; Adipose Tissue; Analysis; Animals; Autografts; Autologous adipose-derived stem cells; BMP7-Smad5 signaling pathway; Bone Morphogenetic Protein 7 - genetics; CD105 antigen; CD29 antigen; CD73 antigen; Cell culture; Cellular signal transduction; Disease; Endometrium; Experiments; Female; Fertility; Fibrosis; Fistula; Flow cytometry; Glands; Health aspects; Humans; Hydrogels; Hyperplasia; Infertility; Intrauterine adhesion; Laboratory animals; Mice; Phosphorylation; Polymerase chain reaction; ShakeGel™3D; Signal Transduction; Smad5 Protein; Stem cell transplantation; Stem Cells; Surgery; Tissue Adhesions; Transplantation; Uterine Diseases; Uterus
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-021-02258-0

The objective was to explore the therapeutic effect of autologous adipose-derived stem cells (ADSCs) combined with ShakeGel™3D transplantation to activate the BMP7-Smad5 signaling pathway to treat intrauterine adhesions (IUA). Autologous ADSCs were isolated and then merged with ShakeGel™3D. The IUA model was established by mechanical injury. The third generation of autologous ADSCs was injected directly into the uterus in combination with ShakeGel™3D. After 7 days of treatment, endometrial morphology, number of endometrial glands, endometrial fibrosis area, and fibrosis biomarker analysis by RT-PCR and IHC were examined. BMP7 and phosphorylation of Smad5 were also detected, and the recovery of infertility function in treated mice was evaluated. Fluorescence-activated cell sorting (FACS) showed that autologous ADSCs expressed CD105 (99.1%), CD29 (99.6%), and CD73 (98.9%). Autologous ADSCs could still maintain a good growth state in ShakeGel™3D. Histological examination revealed that the number of endometrial glands increased significantly, and the area of fibrosis decreased. At the same time, the expression of BMP7 and Smad5 in the ADSCs + Gel group was significantly upregulated, and the final reproductive function of this group was partly recovered. Autologous ADSCs can be used in combination with ShakeGel™3D to maintain functionality and create a viable three-dimensional growth environment. The combined transplantation of autologous ADSCs and ShakeGel™3D promotes the recovery of damaged endometrial tissue by increasing BMP7-Smad5 signal transduction, resulting in endometrium thickening, increased number of glands, and decreased fibrosis, leading to restoration of partial fertility.