Modelling of Cetylpyridinium Chloride Availability in Complex Mixtures for the Prediction of Anti-Microbial Activity Using Diffusion Ordered Spectroscopy, Saturation Transfer Difference and 1D NMR

A range of NMR techniques, including diffusion ordered spectroscopy (DOSY) were used to characterise complex micelles formed by the anti-microbial cationic surfactant cetylpyridium chloride and to quantify the degree of interaction between cetylpyridium chloride and hydroxyethyl cellulose in a varie...

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Bibliographic Details
Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-11, Vol.17 (12), p.1570
Main Authors: Robertson, Cameron, Batabyal, Sayoni, Whitworth, Darren, Coban, Tomris, Smith, Angharad, Montesanto, Alessandra, Lucas, Robert, Le Gresley, Adam
Format: Article
Language:English
Subjects:
Alcohol
anti-microbial
Antimicrobial agents
Biofilms
Cellulose
cetylpyridinium chloride
Copolymers
diffusion
formulation
micelles
NMR
Oral hygiene
Product development
Spectrum analysis
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Summary:A range of NMR techniques, including diffusion ordered spectroscopy (DOSY) were used to characterise complex micelles formed by the anti-microbial cationic surfactant cetylpyridium chloride and to quantify the degree of interaction between cetylpyridium chloride and hydroxyethyl cellulose in a variety of commercially relevant formulations as a model for the disk retention assay. This NMR-derived binding information was then compared with the results of formulation analysis by traditional disk retention assay (DRA) and anti-microbial activity assays to assess the suitability of these NMR techniques for the rapid identification of formulation components that could augment or retard antimicrobial activity DRA. NMR showed a strong ability to predict anti-microbial activity for a diverse range of formulations containing cetylpyridinium chloride (CPC). This demonstrates the value of this NMR-based approach as a rapid, relatively non-destructive method for screening commercial experimental anti-microbial formulations for efficacy and further helps to understand the interplay of excipients and active ingredients.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph17121570