Evaluation of Cytotoxicity and Oxidative Stress of Whole Aerosol from Vuse Alto ENDS Products

Assessment of in vitro cytotoxicity is an important component of tobacco product toxicological evaluations. However, current methods of regulatory testing involve exposing monolayer cell cultures to various preparations of aerosols from cigarettes or other emerging products such as electronic nicoti...

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Bibliographic Details
Published in:Toxics (Basel) 2024-02, Vol.12 (2), p.129
Main Authors: Keyser, Brian M, Leverette, Robert, Wertman, John, Shutsky, Tom, McRae, Reagan, Szeliga, Ken, Makena, Patrudu, Jordan, Kristen
Format: Article
Language:English
Subjects:
Aerosols
air–liquid interface
Batteries
Cell culture
Cell death
Cell-mediated cytotoxicity
Chemical properties
Chronic obstructive pulmonary disease
cigarette smoke
Cigarettes
Cytotoxicity
Dosimetry
Electronic cigarettes
EpiAirway
Evaluation
Exposure
Health aspects
Menthol
Nicotine
NRF2 protein
Oxidative stress
Physiological aspects
Reporter gene
Smoking
Test systems
Three dimensional models
Tobacco
Toxicity
Two dimensional models
Vuse Alto ENDS
whole aerosol
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Summary:Assessment of in vitro cytotoxicity is an important component of tobacco product toxicological evaluations. However, current methods of regulatory testing involve exposing monolayer cell cultures to various preparations of aerosols from cigarettes or other emerging products such as electronic nicotine delivery systems (ENDS), which are not representative of human exposure. In the present study, a whole aerosol (WA) system was used to expose lung epithelial cultures (2D and 3D) to determine the potential of six Vuse Alto ENDS products that varied in nicotine content (1.8%, 2.4%, and 5%) and flavors (Golden Tobacco, Rich Tobacco, Menthol, and Mixed Berry), along with a marketed ENDS and a marked cigarette comparator to induce cytotoxicity and oxidative stress. The WA from the Vuse Alto ENDS products was not cytotoxic in the NRU and MTT assays, nor did it activate the Nrf2 reporter gene, a marker of oxidative stress. In summary, Vuse Alto ENDS products did not induce cytotoxic or oxidative stress responses in the in vitro models. The WA exposures used in the 3D in vitro models described herein may be better suited than 2D models for the determination of cytotoxicity and other in vitro functional endpoints and represent alternative models for regulatory evaluation of tobacco products.
ISSN:2305-6304
2305-6304
DOI:10.3390/toxics12020129