Exogenous auxin regulates the growth and development of peach fruit at the expansion stage by mediating multiple-hormone signaling

• Published in: BMC plant biology 2023-10, Vol.23 (1), p.1-499, Article 499
• Main Authors: Zhang, Yanping, Su, Ziwen, Luo, Linjia, Wang, Pengkai, Zhu, Xudong, Liu, Jiecai, Wang, Chen
• Format: Article
• Language: English
• Subjects: Abscisic acid; Auxin; Auxin; peach; Auxins; Biosynthesis; Cell division; Cell walls; Cellular signal transduction; Chromosome 7; Crop yield; DNA binding proteins; Fruit expansion stage; Fruits; Genes; Genetic transcription; Growth; Hormones; Metabolism; Multiple-hormone signaling; Peach; Phenylalanine; Physiological aspects; Plant growth; Plant hormones; Regulatory mechanisms (biology); Signal transduction; Sucrose; Transcription factors
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-023-04514-2

Fruit expansion stage is crucial to fruit yield and quality formation, and auxin plays a significant role by mediating multi-hormone signals during fruit expansion. However, till now, it is still unclear of the molecular regulatory network during auxin-mediated peach fruit expansion. Here, exogenous NAA application markedly increased IAA content and drastically decreased ABA content at the fruit expansion stage. Correspondingly, NAA mainly induced the auxin biosynthesis gene (1 PpYUCCA) and early auxin-responsive genes (7PpIAA, 3 PpGH3, and 14 PpSAUR); while NAA down-regulated ABA biosynthesis genes (2 PpNCED, 1 PpABA3, and 1 PpAAO3). In addition, many DEGs involved in other plant hormone biosynthesis and signal transduction were significantly enriched after NAA treatment, including 7 JA, 7 CTK, 6 ETH, and 3 GA. Furthermore, we also found that NAA treatment down-regulated most of genes involved in the growth and development of peach fruit, including the cell wall metabolism-related genes (PpEG), sucrose metabolism-related genes (PpSPS), phenylalanine metabolism-related genes (PpPAL, Pp4CL, and PpHCT), and transcription factors (PpNAC, PpMADS-box, PpDof, PpSBP, and PpHB). Overall, NAA treatment at the fruit expansion stage could inhibit some metabolism processes involved in the related genes in the growth and development of peach fruit by regulating multiple-hormone signaling networks. These results help reveal the short-term regulatory mechanism of auxin at the fruit expansion stage and provide new insights into the multi-hormone cascade regulatory network of fruit growth and development.