Mortality and immune challenge of a native isolate of Beauveria bassina against the larvae of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Background Entomopathogenic fungi (EPF) attack a wide range of insects. They are considered environmental friendly alternatives to synthetic insecticides for pest control. In the present study, virulence of a native isolate of the EPF, Beauveria bassiana Vuillemin (Hypocreales: Cordycipitaceae) was...

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Published in:Egyptian journal of biological pest control 2021-02, Vol.31 (1), p.1-9, Article 37
Main Authors: Pour, Sarah Aghaee, Zibaee, Arash, Rostami, Maryam Gohar, Hoda, Hassan, Shahriari, Morteza
Format: Article
Language:English
Subjects:
Beauveria bassiana
Bioassays
Biomedical and Life Sciences
Conidia
Entomopathogenic fungi
Glyphodes pyloalis
Hemocytes
Immune response
Immune system
Immunity
Injection
Insecticides
Insects
Larvae
Lepidoptera
Leukocytes (granulocytic)
Life Sciences
Mycoinsecticides
Nodules
Pest control
Phenoloxidase
Plasmatocytes
Spores
Virulence
Zoology
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Summary:Background Entomopathogenic fungi (EPF) attack a wide range of insects. They are considered environmental friendly alternatives to synthetic insecticides for pest control. In the present study, virulence of a native isolate of the EPF, Beauveria bassiana Vuillemin (Hypocreales: Cordycipitaceae) was evaluated against the least mulberry pyralid, Glyphodes pyloalis Walker (Lepidoptera: Crambidae), through bioassay, pathogenic pathways, and immune responses. Results The values of 2.6 × 10 4 conidia/ml and 3.54 days were determined as the median lethal concentration (LC 50 ) and median lethal concentration (LT 50 ) of AM-118 against the 4th instar larvae of G. pyloalis , respectively. The activities of proteases and chitinases in the culture medium containing the larval cuticle were higher than the control medium. Moreover, the total and the differential hemocyte counts of the larvae were significantly changed after injection with AM-118 spores. The highest numbers of total hemocytes and granulocytes were obtained 3 and 6 h post-injection, while the highest numbers of plasmatocytes and nodules were observed 6 h post-injection. The highest activity of phenoloxidase was determined 12 h post-injection by AM-118 spores . Conclusions The findings imply on virulence of the AM-118 isolate against the larvae of G. pyoalis although immune responses were triggered by the spores.
ISSN:2536-9342
1110-1768
2536-9342
DOI:10.1186/s41938-021-00386-6