The Transcriptomic Response of the Boll Weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), following Exposure to the Organophosphate Insecticide Malathion

• Published in: Insects (Basel, Switzerland) Switzerland), 2023-02, Vol.14 (2), p.197
• Main Authors: Perkin, Lindsey C, Cohen, Zachary P, Carlson, Jason W, Suh, Charles P-C
• Format: Article
• Language: English
• Subjects: ABC transporters; Acetylcholinesterase; Adaptation; Anthonomus grandis grandis; boll weevil; Boll weevils; Coleoptera; Communication; Corrosion resistance; Cotton; Curculionidae; Detoxification; Environmental conditions; Enzymes; Eradication; Esterase; Exposure; Gene amplification; Gene duplication; Gene expression; Gene frequency; Genomes; Glutathione; Insecticide resistance; Insecticides; Insects; Isoforms; Malathion; Mosquitoes; Mutation; Nucleotide sequence; Nucleotides; Organophosphates; Pest resistance; Pesticides; Pests; Polymorphism; Ribonucleic acid; RNA; RNA-seq; Single-nucleotide polymorphism; Transcriptomics
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects14020197

Insecticide tolerance and resistance have evolved countless times in insect systems. Molecular drivers of resistance include mutations in the insecticide target site and/or gene duplication, and increased gene expression of detoxification enzymes. The boll weevil, Boheman (Coleoptera: Curculionidae), is a pest of commercial cotton and has developed resistance in the field to several insecticides; however, the current organophosphate insecticide, malathion, used by USA eradication programs remains effective despite its long-term use. Here, we present findings from an RNA-seq experiment documenting gene expression post-exposure to field-relevant concentrations of malathion, which was used to provide insight on the boll weevil's continued susceptibility to this insecticide. Additionally, we incorporated a large collection of boll weevil whole-genome resequencing data from nearly 200 individuals collected from three geographically distinct areas to determine SNP allele frequency of the malathion target site, as a proxy for directional selection in response to malathion exposure. No evidence was found in the gene expression data or SNP data consistent with a mechanism of enhanced tolerance or resistance adaptation to malathion in the boll weevil. Although this suggests continued effectiveness of malathion in the field, we identified important temporal and qualitative differences in gene expression between weevils exposed to two different concentrations of malathion. We also identified several tandem isoforms of the detoxifying esterase B1 and glutathione S-transferases, which are putatively associated with organophosphate resistance.