Molecular Characterization of Fasciola hepatica in Sheep Based on DNA Sequences of Ribosomal ITS-1

Introduction: World Health Organization (WHO) considers Fascioliasis as a neglected tropical disease that requires global efforts for disease control. Data from the genetic characterization of Fasciola population shed light on the spread of infections among animals which could help in the developmen...

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Bibliographic Details
Published in:Infection and drug resistance 2023-10, Vol.16, p.6661-6671
Main Authors: Alsulami, Muslimah N, Mohamed, Khalil, Wakid, Majed H, Abdel-Gaber, Rewaida, Timsah, Ashraf G, Al-Megrin, Wafa Abdullah I, Khan, Adil, Elkholy, Walaa A, Abdelaal, Khaled A.A, Elshabrawy, Hatem A, Kady, Asmaa M. El
Format: Article
Language:English
Subjects:
characterization
Development and progression
Disease transmission
DNA
DNA sequencing
fasciola hepatica
Genetic aspects
genetic diversity
Health aspects
Nucleotide sequencing
Original Research
Parasitic diseases
Phylogeny
sheep
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Summary:Introduction: World Health Organization (WHO) considers Fascioliasis as a neglected tropical disease that requires global efforts for disease control. Data from the genetic characterization of Fasciola population shed light on the spread of infections among animals which could help in the development of effective parasite control. The aim of the present work was to genetically characterize Fasciola adult worms isolated from sheep in Saudi Arabia by sequence analysis of ITS-1 region. Methods: A total of 12,653 slaughtered sheep in Jeddah city, Saudi Arabia were examined for the presence of Fasciola spp. adult worms. The ITS-1 region of all parasites was amplified and sequenced. Results: Overall, 12 variants DNA sequences were obtained. The variance of isolates ranged from 0.00771 to 0.34405. BLAST search showed that all obtained sequences were Fasciola hepatica and had >99.3% similarity with F. hepatica isolates from Spain and USA (from different hosts other than sheep). Phylogenetic analysis showed that Fasciola isolates were closely related to isolates from different countries. Discussion: The current study showed that F. hepatica was the only spp. isolated from sheep in Jeddah. Further studies from different localities in Saudi Arabia are needed to help in the development of disease control. Keywords: genetic diversity, characterization, Fasciola hepatica, parasitic diseases, sheep
ISSN:1178-6973
1178-6973
DOI:10.2147/IDR.S421206