Biomolecular Clusters Distribution up to Mega Dalton Region Using MALDI-Quadrupole Ion Trap Mass Spectrometer

We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio ( / ) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MA...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-09, Vol.19 (9), p.2789
Main Authors: Chuang, Yung-Kun, Lai, Szu-Hsueh, Lin, Jung-Lee, Chen, Chung-Hsuan
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biomolecules
Bovine serum albumin
bovine serum albumin (BSA)
Cattle
Cluster Analysis
Cytochrome c
Cytochromes c - chemistry
Efficiency
Equipment Design
Hypotheses
Ionization
Ions
large clusters
Laser ablation
Lasers
MALDI
Mass spectrometry
Molecular weight
Quadrupoles
quantitative distribution
Scientific imaging
Sensors
Serum albumin
Serum Albumin, Bovine - chemistry
Size distribution
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:We present the first report on complete cluster distributions of cytochrome c (molecular weight of 12.4 kDa) and bovine serum albumin ((BSA), molecular weight of 66.4 kDa) with mass-to-charge ratio ( / ) reaching 350,000 and 1,400,000, respectively, by matrix-assisted laser desorption/ionization (MALDI). Large cluster distributions of the analytes were measured by our homemade frequency-scanned quadrupole ion trap (QIT) mass spectrometer with a charge detector. To our knowledge, we report the highest / clusters of these two biomolecules. The quantitative results indicate that large clusters ions of cytochrome c and BSA follow the power law ( ² > 0.99) with cluster size distribution, which provides experimental evidence for the laser ablation studies of MALDI.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19092789