Effect of Blue LED Light on Bioemulsifier Production in Bioreactor by Aureobasidium pullulans LB83 in Solid State Fermentation

• Published in: Fermentation (Basel) 2023-11, Vol.9 (11), p.946
• Main Authors: Rubio-Ribeaux, Daylin, Costa, Rogger Alessandro Mata da, Pereira, Renan Murbach, Marcelino, Paulo Ricardo Franco, Casciatori, Fernanda Perpétua, Santos, Júlio César dos, Silva, Silvio Silvério da
• Format: Article
• Language: English
• Subjects: Aureobasidium pullulans; Bagasse; Biomass; Bioreactors; cellulases; Enzymatic activity; Enzymes; Fermentation; Humidity; Kerosene; LED light-assisted process; Light; Metabolism; Metabolites; Microorganisms; Scanning electron microscopy; Solid state fermentation; Soybeans; Sugarcane; sugarcane bagasse; surface active compounds; Textile fibers; Vegetable oils; Yeast
• ISSN: 2311-5637; 2311-5637
• DOI: 10.3390/fermentation9110946

This study analyzed the impact of LED light on bioemulsifier production by Aureobasidium pullulans LB83 in solid-state fermentation (SSF) using pre-treated sugarcane bagasse (PSB). The biomass was subjected to alkaline pre-treatment and conducted fermentations in Erlenmeyer flasks containing 2 g of PSB that were immersed in a humectant solution with a cell concentration of 108 cells/mL. The screening involved varying LED light wavelengths (green, red, orange, and blue) over a 7-day period at 28 °C. Notably, under the influence of blue light, the process achieved maximum production, yielding an EI24% of 63.9% and 45.1% for soybean oil and kerosene, respectively. Prolonged exposure to blue light for 11 days at 28 °C resulted in maximum bioemulsifier production (75%) and cellulolytic enzyme activity (3.67 IU g−1 for endoglucanase and 0.41 IU g−1 for exoglucanase) with soybean oil and kerosene. Experiments in a bioreactor, with varying light conditions (dark, white light, and blue LED light), demonstrated that the blue LED bioreactor outperformed others, achieving EI24% values of 55.0% and 45.7% for soybean oil and kerosene, respectively. The scanning electron microscopy (SEM) confirmed yeast growth under these conditions after 9 days. Our findings highlight the significant potential of LED light to enhance bioemulsifier production by A. pullulans LB83 from PSB.