Time-Course Transcriptome Landscape of Bursa of Fabricius Development and Degeneration in Chickens

The bursa of Fabricius (BF) is a target organ for various pathogenic microorganisms; however, the genes that regulate BF development and decline have not been fully characterized. Therefore, in this study, histological sections of the BF were obtained from black-boned chickens at 7 (N7), 42 (N42), 9...

Full description

Saved in:
Bibliographic Details
Published in:Agriculture (Basel) 2022-08, Vol.12 (8), p.1194
Main Authors: Huang, Lan, Hu, Yaodong, Guo, Qixin, Chang, Guobin, Bai, Hao
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Atrophy
B cells
Bioinformatics
Biological activity
Birds
Bursa of Fabricius
chicken
Chickens
Cluster analysis
Degeneration
degradation
development
Disease
Gene expression
Genes
Lymphocytes
Microorganisms
Polymerase chain reaction
Poultry
Software
Transcriptomes
transcriptomic analysis
Transcriptomics
Variance analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The bursa of Fabricius (BF) is a target organ for various pathogenic microorganisms; however, the genes that regulate BF development and decline have not been fully characterized. Therefore, in this study, histological sections of the BF were obtained from black-boned chickens at 7 (N7), 42 (N42), 90 (N90) and 120 days (N120) of age, and the differential expression and expression trends of the BF at different stages were analyzed by transcriptome analysis. The results showed that the growth of the BF progressively matured with age, followed by gradual shrinkage and disappearance. Transcriptome differential analysis revealed 5914, 5513, 4575, 577, 530 and 66 differentially expressed genes (DEG) in six different comparison groups: N7 vs. N42, N7 vs. N90, N7 vs. N120, N42 vs. N90, N42 vs. N120 and N90 vs. N120, respectively. Moreover, we performed transcriptomic analysis of the time series of BF development and identified the corresponding stages of biological process enrichment. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression of the 16 DEGs during bursal growth and development. These results were consistent with the transcriptome results, indicating that they reflect the expression of the BF during growth and development and that these genes reflect the characteristics of the BF at different times of development and decline. These findings reflect the characteristics of the BF at different time intervals.
ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture12081194