Complete Chloroplast Genome of Pinus densiflora Siebold & Zucc. and Comparative Analysis with Five Pine Trees

Pinus densiflora (Korean red pine) is widely distributed in East Asia and considered one of the most important species in Korea. In this study, the complete chloroplast genome of P. densiflora was sequenced by combining the advantages of Oxford Nanopore MinION and Illumina MiSeq. The sequenced genom...

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Bibliographic Details
Published in:Forests 2019-07, Vol.10 (7), p.600
Main Authors: Kang, Hye-In, Lee, Hyun Oh, Lee, Il Hwan, Kim, In Sik, Lee, Seok-Woo, Yang, Tae Jin, Shim, Donghwan
Format: Article
Language:English
Subjects:
chloroplast
Chloroplasts
Comparative analysis
Cultural heritage
Error correction & detection
Genes
Genetic engineering
genome
Genomes
Inverted repeat
MiSeq
Ndh gene
Nucleotide sequence
Oxford Nanopore Technology (ONT)
Phylogenetics
Phylogeny
Pine
pine tree
Pine trees
Pinus densiflora
Proteins
Software
Species
Tuna
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Summary:Pinus densiflora (Korean red pine) is widely distributed in East Asia and considered one of the most important species in Korea. In this study, the complete chloroplast genome of P. densiflora was sequenced by combining the advantages of Oxford Nanopore MinION and Illumina MiSeq. The sequenced genome was then compared with that of a previously published conifer plastome. The chloroplast genome was found to be circular and comprised of a quadripartite structure, including 113 genes encoding 73 proteins, 36 tRNAs and 4 rRNAs. It had short inverted repeat regions and lacked ndh gene family genes, which is consistent with other Pinaceae species. The gene content of P. densiflora was found to be most similar to that of P. sylvestris. The newly attempted sequencing method could be considered an alternative method for obtaining accurate genetic information, and the chloroplast genome sequence of P. densiflora revealed in this study can be used in the phylogenetic analysis of Pinus species.
ISSN:1999-4907
1999-4907
DOI:10.3390/f10070600