In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study

• Published in: Stem cell research & therapy 2017-10, Vol.8 (1), p.218-218, Article 218
• Main Authors: Bearden, Robert N, Huggins, Shannon S, Cummings, Kevin J, Smith, Roger, Gregory, Carl A, Saunders, William B
• Format: Article
• Language: English
• Subjects: Adipogenesis - genetics; Adipose tissue; Adipose Tissue - cytology; Adipose Tissue - metabolism; Adipose tissues; Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Arthritis; Body fat; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - genetics; Bone Morphogenetic Protein 2 - metabolism; Bone morphogenetic proteins; Canine; CD105 antigen; CD34 antigen; CD44 antigen; CD45 antigen; CD9 antigen; CD90 antigen; Cell Proliferation; Characterization; Chondrogenesis - genetics; Collagen (type II); Collagen Type II - genetics; Collagen Type II - immunology; Cytokines; Differentiation; Dogs; Female; Flow cytometry; Gene Expression; Growth factors; Immunomodulation; Interleukin 6; Interleukin-6 - genetics; Interleukin-6 - immunology; Ligaments; Lipopolysaccharides; Macrophages; Male; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Multipotent Stem Cells - cytology; Multipotent Stem Cells - metabolism; Multipotent stromal cells; Nanog Homeobox Protein - genetics; Nanog Homeobox Protein - metabolism; Oct-4 protein; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Organ Specificity; Osteogenesis; Osteogenesis - genetics; Plastics; Pluripotency; Polymerase chain reaction; Primary Cell Culture; Proteoglycans - genetics; Proteoglycans - immunology; Rodents; Skin & tissue grafts; Skull; SOXB1 Transcription Factors - genetics; SOXB1 Transcription Factors - metabolism; Stem cells; Stromal cells; Studies; Supplements; Synovial Membrane - cytology; Synovial Membrane - metabolism; Synovium; Tissue Donors; Translation; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-017-0639-6

The dog represents an excellent large animal model for translational cell-based studies. Importantly, the properties of canine multipotent stromal cells (cMSCs) and the ideal tissue source for specific translational studies have yet to be established. The aim of this study was to characterize cMSCs derived from synovium, bone marrow, and adipose tissue using a donor-matched study design and a comprehensive series of in-vitro characterization, differentiation, and immunomodulation assays. Canine MSCs were isolated from five dogs with cranial cruciate ligament rupture. All 15 cMSC preparations were evaluated using colony forming unit (CFU) assays, flow cytometry analysis, RT-PCR for pluripotency-associated genes, proliferation assays, trilineage differentiation assays, and immunomodulation assays. Data were reported as mean ± standard deviation and compared using repeated-measures analysis of variance and Tukey post-hoc test. Significance was established at p