Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

• Published in: International journal of molecular sciences 2020-08, Vol.21 (16), p.5899
• Main Authors: Augustyniak, Adam, Pawłowicz, Izabela, Lechowicz, Katarzyna, Izbiańska-Jankowska, Karolina, Arasimowicz-Jelonek, Magdalena, Rapacz, Marcin, Perlikowski, Dawid, Kosmala, Arkadiusz
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Accumulation; antioxidant system; Antioxidants; Calvin cycle; Cell membranes; Chlorophyll; Chloroplasts; Cold; Cold acclimation; Cold tolerance; Enzymes; Festuca - genetics; Festuca - metabolism; Fluorescence; forage grasses; Freezing; freezing tolerance; Gas exchange; Gene expression; Genetic Introgression; Grasses; Homeostasis; Kinases; Lipid peroxidation; Lipids; Lolium - genetics; Lolium - metabolism; Lolium-Festuca; Low temperature; Membranes; Mitochondria; Photosynthesis; Photosynthesis - genetics; Photosynthetic apparatus; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Transcription factors; Transcriptome; Water use
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21165899

Though winter-hardiness is a complex trait, freezing tolerance was proved to be its main component. Species from temperate regions acquire tolerance to freezing in a process of cold acclimation, which is associated with the exposure of plants to low but non-freezing temperatures. However, mechanisms of cold acclimation in grasses, important for forage production in Europe, have not been fully recognized. Thus, two introgression forms with distinct freezing tolerance were used herein as models in the comprehensive research to dissect these mechanisms in that group of plants. The work was focused on: (i) analysis of cellular membranes' integrity; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gas exchange; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); (iii) analysis of plant antioxidant capacity (reactive oxygen species generation; gene expression, protein accumulation, and activity of selected enzymes); and (iv) analysis of Cor14b accumulation, under cold acclimation. The more freezing tolerant introgression form revealed a higher integrity of membranes, an ability to cold acclimate its photosynthetic apparatus and higher water use efficiency after three weeks of cold acclimation, as well as a higher capacity of the antioxidant system and a lower content of reactive oxygen species in low temperature.