Molecular analysis of sarcoidosis tissues for mycobacterium species DNA

We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis and on 25 control tissue specimens consisting of mediastinal or cervical lymph nodes and lung biopsies. Mycobacterium species 16S rRN...

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Bibliographic Details
Published in:Emerging infectious diseases 2002-11, Vol.8 (11), p.1334-1341
Main Authors: Drake, Wonder Puryear, Pei, Zhiheng, Pride, David T, Collins, Robert D, Cover, Timothy L, Blaser, Martin J
Format: Article
Language:English
Subjects:
16S rRNA
Adolescent
Adult
Aged
Aged, 80 and over
DNA
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Female
Genes, Bacterial - genetics
Humans
IS6110
Lymph Nodes - microbiology
Male
Middle Aged
Mycobacteria
Mycobacterium
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium - isolation & purification
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Physiological aspects
Polymerase Chain Reaction
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - genetics
rpoB
Sarcoidosis
Sarcoidosis - microbiology
Sensitivity and Specificity
Species Specificity
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Summary:We performed polymerase chain reaction analysis, for Mycobacterium species 16S rRNA, rpoB, and IS6110 sequences, on 25 tissue specimens from patients with sarcoidosis and on 25 control tissue specimens consisting of mediastinal or cervical lymph nodes and lung biopsies. Mycobacterium species 16S rRNA sequences were amplified from 12 (48%) rpoB sequences and from 6 (24%) of the sarcoidosis specimens. In total, 16S rRNA or rpoB sequences were amplified from 15 sarcoidosis specimens (60%) but were not detected in any of the control tissues (p=0.00002, chi square). In three specimens, the sequences resembled Mycobacterium species other than M. tuberculosis. All specimens with sequences consistent with M. tuberculosis were negative for IS6110. We provide evidence that one of a variety of Mycobacterium species, especially organisms resembling M. tuberculosis, is found in most patients with sarcoidosis.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid0811.020318