Multiple drug-susceptibility screening in Mycobacterium bovis: new nucleotide polymorphisms in the embB gene among ethambutol susceptible strains

Highlights • We assessed animal Mycobacteriumbovis susceptibility to six antitubercular drugs. • We used the standard method and a simplified, dichotomous REMA(d-REMA) method. • There was good agreement between the d-REMA assay and the reference method. • Four new single-nucleotide polymorphisms wer...

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Bibliographic Details
Published in:International journal of infectious diseases 2015-04, Vol.33 (C), p.39-44
Main Authors: Marianelli, Cinzia, Armas, Federica, Boniotti, Maria Beatrice, Mazzone, Piera, Pacciarini, Maria Lodovica, Di Marco Lo Presti, Vincenzo
Format: Article
Language:English
Subjects:
Animals
Antimicrobial resistance
Antitubercular Agents - pharmacology
Bovine tuberculosis
Cattle
d-REMA
Drug Resistance, Multiple, Bacterial - genetics
Ethambutol - pharmacology
Genes, Bacterial
Infectious Disease
Mutation
Mycobacterium bovis
Mycobacterium bovis - drug effects
Mycobacterium bovis - genetics
Mycobacterium bovis - isolation & purification
Pentosyltransferases - genetics
Polymorphism, Genetic
Pulmonary/Respiratory
Resazurin microtitre assay
Sheep - microbiology
Swine - microbiology
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Summary:Highlights • We assessed animal Mycobacteriumbovis susceptibility to six antitubercular drugs. • We used the standard method and a simplified, dichotomous REMA(d-REMA) method. • There was good agreement between the d-REMA assay and the reference method. • Four new single-nucleotide polymorphisms were identified in the embB gene. • No association was found between embB genetic profiles and EMB resistance results.
ISSN:1201-9712
1878-3511
DOI:10.1016/j.ijid.2014.12.043