Investigation of Potential cGMP-Specific PDE V and Aminopeptidase N Inhibitors of Allium ampeloprasum L. and Its Bioactive Components: Kinetic and Molecular Docking Studies

• Published in: International journal of molecular sciences 2023-09, Vol.24 (17), p.13319
• Main Authors: Choi, Jun-Hui, Park, Seung-Man, Kim, Seung
• Format: Article
• Language: English
• Subjects: Allium ampeloprasum L; aminopeptidase N; Cardiomyocytes; cGMP-specific PDE V; Endocrine system; Enzymes; Erectile dysfunction; Garlic; Heart; kinetics; molecular docking; Nitric oxide; Peptides; Smooth muscle; Testosterone
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241713319

The primary objectives of this study were to assess the inhibitory effects of Allium ampeloprasum L. extract (AAE) and its derived organosulfur and polyphenolic compounds on the enzymatic activities of cGMP-specific PDE V (PDE5) and aminopeptidase N (APN). Additionally, the study aimed to investigate their potential as inhibitors against these two target enzymes through kinetic analyses and molecular docking studies. The in vitro enzyme assays demonstrated that both AAE and its derived compounds significantly decreased the activity of PDE5 and APN. Further analyses involving kinetics and molecular docking provided insights into the specific inhibitor types of AAE and its derived compounds along with the proposed molecular docking models illustrating the interactions between the ligands (the compounds) and the enzymes (PDE5 and APN). In particular, AAE-derived polyphenolic compounds showed relatively stable binding affinity (−7.2 to −8.3 kcal/mol) on PDE5 and APN. Our findings proved the potential as an inhibitor against PDE5 and APN of AAE and AAE-derived organosulfur and polyphenolic compounds as well as a functional material for erectile dysfunction improvement.