Validation of mathematical model with phosphate activation effect by batch (R)-phenylacetylcarbinol biotransformation process utilizing Candida tropicalis pyruvate decarboxylase in phosphate buffer

The ( R )-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/12...

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Published in:Scientific reports 2021-06, Vol.11 (1), p.11813-11813, Article 11813
Main Authors: Khemacheewakul, Julaluk, Taesuwan, Siraphat, Nunta, Rojarej, Techapun, Charin, Phimolsiripol, Yuthana, Rachtanapun, Pornchai, Jantanasakulwong, Kittisak, Porninta, Kritsadaporn, Sommanee, Sumeth, Mahakuntha, Chatchadaporn, Chaiyaso, Thanongsak, Seesuriyachan, Phisit, Reungsang, Alissara, Trinh, Ngoc Thao Ngan, Wangtueai, Sutee, Sommano, Sarana Rose, Leksawasdi, Noppol
Format: Article
Language:English
Subjects:
631/326
631/326/2522
631/61
639/166
639/166/898
Benzaldehyde
Biotransformation
Candida tropicalis
Differential equations
Humanities and Social Sciences
Kinetics
Mathematical models
multidisciplinary
Ordinary differential equations
Pyruvate decarboxylase
Pyruvic acid
Science
Science (multidisciplinary)
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Summary:The ( R )-phenylacetylcarbinol (PAC) batch biotransformation kinetics for partially purified Candida tropicalis TISTR 5350 pyruvate decarboxylase (PDC) were determined to validate a comprehensive mathematical model in 250 mL scale with 250 mM phosphate buffer/pH 7.0. PDC could convert initial 100/120 mM benzaldehyde/pyruvate substrates to the statistical significantly highest ( p  ≤ 0.05) maximum PAC concentration (95.8 ± 0.1 mM) and production rate (0.639 ± 0.001 mM min −1 ). A parameter search strategy aimed at minimizing overall residual sum of square (RSS T ) based on a system of six ordinary differential equations was applied to PAC biotransformation profiles with initial benzaldehyde/pyruvate concentration of 100/120 and 30/36 mM. Ten important biotransformation kinetic parameters were then elucidated including the zeroth order activation rate constant due to phosphate buffer species ( k a ) of (9.38 ±  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91294-0