Effects of arecoline on cell growth, migration, and differentiation in cementoblasts

Abstract Background/purpose Studies have supported a higher prevalence of periodontal disease among areca quid chewers than non-chewers. However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, th...

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Published in:Journal of dental sciences 2015-12, Vol.10 (4), p.388-393
Main Authors: Chen, Yi-Juai, Lee, Shiuan-Shinn, Huang, Fu-Mei, Yu, Hui-Chieh, Tsai, Chi-Cheng, Chang, Yu-Chao
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Language:English
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Advanced Basic Science
arecoline
cementoblasts
cytotoxicity
Dentistry
differentiation
migration
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container_title Journal of dental sciences
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creator Chen, Yi-Juai
Lee, Shiuan-Shinn
Huang, Fu-Mei
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description Abstract Background/purpose Studies have supported a higher prevalence of periodontal disease among areca quid chewers than non-chewers. However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, the major alkaloid of areca nut, on murine immortalized cementoblast cell line (OCCM.30). Materials and methods Cytotoxicity was judged using tetrazolium bromide reduction assay. Cell migration was evaluated by Transwell assay. In vitro mineral nodule formation was assayed by von Kossa staining. Cell differentiation was examined by alkaline phosphatase activity with substrate assay. The production of osteoprotegerin was evaluated using enzyme-linked immunosorbent assay. Results Arecoline demonstrated cytotoxicity to cementoblasts in a dose-dependent and time-dependent manner (P 
doi_str_mv 10.1016/j.jds.2015.04.002
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However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, the major alkaloid of areca nut, on murine immortalized cementoblast cell line (OCCM.30). Materials and methods Cytotoxicity was judged using tetrazolium bromide reduction assay. Cell migration was evaluated by Transwell assay. In vitro mineral nodule formation was assayed by von Kossa staining. Cell differentiation was examined by alkaline phosphatase activity with substrate assay. The production of osteoprotegerin was evaluated using enzyme-linked immunosorbent assay. Results Arecoline demonstrated cytotoxicity to cementoblasts in a dose-dependent and time-dependent manner (P &lt; 0.05). Arecoline attenuated cell migration in a dose-dependent manner (P &lt; 0.05). Arecoline treatment markedly suppressed cementoblast-mediated biomineralization in vitro compared to untreated cells at Day 8. Arecoline was found to inhibit alkaline phosphatase activity in a time-dependent manner (P &lt; 0.05). In addition, arecoline decreased the secretion of osteoprotegerin in a dose-dependent manner (P &lt; 0.05). Conclusion Taken together, these results suggest that arecoline could inhibit cell growth, migration, and differentiation in cementoblasts. Areca quid chewers might be more susceptible to the destruction of periodontium and less responsive to regenerative procedure during periodontal therapy.</description><identifier>ISSN: 1991-7902</identifier><identifier>DOI: 10.1016/j.jds.2015.04.002</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Advanced Basic Science ; arecoline ; cementoblasts ; cytotoxicity ; Dentistry ; differentiation ; migration</subject><ispartof>Journal of dental sciences, 2015-12, Vol.10 (4), p.388-393</ispartof><rights>2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c531t-112a84d93847965266bdc1d014c29a59f4ca4f7eedf2c312739110d79721f8503</citedby><cites>FETCH-LOGICAL-c531t-112a84d93847965266bdc1d014c29a59f4ca4f7eedf2c312739110d79721f8503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1991790215000525$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids></links><search><creatorcontrib>Chen, Yi-Juai</creatorcontrib><creatorcontrib>Lee, Shiuan-Shinn</creatorcontrib><creatorcontrib>Huang, Fu-Mei</creatorcontrib><creatorcontrib>Yu, Hui-Chieh</creatorcontrib><creatorcontrib>Tsai, Chi-Cheng</creatorcontrib><creatorcontrib>Chang, Yu-Chao</creatorcontrib><title>Effects of arecoline on cell growth, migration, and differentiation in cementoblasts</title><title>Journal of dental sciences</title><description>Abstract Background/purpose Studies have supported a higher prevalence of periodontal disease among areca quid chewers than non-chewers. However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, the major alkaloid of areca nut, on murine immortalized cementoblast cell line (OCCM.30). Materials and methods Cytotoxicity was judged using tetrazolium bromide reduction assay. Cell migration was evaluated by Transwell assay. In vitro mineral nodule formation was assayed by von Kossa staining. Cell differentiation was examined by alkaline phosphatase activity with substrate assay. The production of osteoprotegerin was evaluated using enzyme-linked immunosorbent assay. Results Arecoline demonstrated cytotoxicity to cementoblasts in a dose-dependent and time-dependent manner (P &lt; 0.05). Arecoline attenuated cell migration in a dose-dependent manner (P &lt; 0.05). Arecoline treatment markedly suppressed cementoblast-mediated biomineralization in vitro compared to untreated cells at Day 8. Arecoline was found to inhibit alkaline phosphatase activity in a time-dependent manner (P &lt; 0.05). In addition, arecoline decreased the secretion of osteoprotegerin in a dose-dependent manner (P &lt; 0.05). Conclusion Taken together, these results suggest that arecoline could inhibit cell growth, migration, and differentiation in cementoblasts. 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However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, the major alkaloid of areca nut, on murine immortalized cementoblast cell line (OCCM.30). Materials and methods Cytotoxicity was judged using tetrazolium bromide reduction assay. Cell migration was evaluated by Transwell assay. In vitro mineral nodule formation was assayed by von Kossa staining. Cell differentiation was examined by alkaline phosphatase activity with substrate assay. The production of osteoprotegerin was evaluated using enzyme-linked immunosorbent assay. Results Arecoline demonstrated cytotoxicity to cementoblasts in a dose-dependent and time-dependent manner (P &lt; 0.05). Arecoline attenuated cell migration in a dose-dependent manner (P &lt; 0.05). Arecoline treatment markedly suppressed cementoblast-mediated biomineralization in vitro compared to untreated cells at Day 8. Arecoline was found to inhibit alkaline phosphatase activity in a time-dependent manner (P &lt; 0.05). In addition, arecoline decreased the secretion of osteoprotegerin in a dose-dependent manner (P &lt; 0.05). Conclusion Taken together, these results suggest that arecoline could inhibit cell growth, migration, and differentiation in cementoblasts. Areca quid chewers might be more susceptible to the destruction of periodontium and less responsive to regenerative procedure during periodontal therapy.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.jds.2015.04.002</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Advanced Basic Science
arecoline
cementoblasts
cytotoxicity
Dentistry
differentiation
migration
title Effects of arecoline on cell growth, migration, and differentiation in cementoblasts
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