Decoy TRAIL receptor CD264: a cell surface marker of cellular aging for human bone marrow-derived mesenchymal stem cells

Mesenchymal stem cells (MSCs) are a mixture of progenitors that are heterogeneous in their regenerative potential. Development of MSC therapies with consistent efficacy is hindered by the absence of an immunophenotype of MSC heterogeneity. This study evaluates decoy TRAIL receptor CD264 as potential...

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Bibliographic Details
Published in:Stem cell research & therapy 2017-09, Vol.8 (1), p.201-201, Article 201
Main Authors: Madsen, Sean D, Russell, Katie C, Tucker, H Alan, Glowacki, Julie, Bunnell, Bruce A, O'Connor, Kim C
Format: Article
Language:English
Subjects:
Adult
Aging
Antigens
Apoptosis
Bone marrow
Bone surgery
Cancer
CD264
Cell cycle
Cell Differentiation
Cell Proliferation
Cell surface
Cells, Cultured
Cellular aging
Cellular proteins
Cellular Senescence
Cyclin-dependent kinase inhibitor p21
Decoy TRAIL receptor
Female
Genetic aspects
Genetic engineering
Health aspects
Heterogeneity
HT29 Cells
Humans
Kinases
Male
MCF-7 Cells
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
Mesenchyme
Middle Aged
p53 Protein
Receptors, TNF-Related Apoptosis-Inducing Ligand - genetics
Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism
Senescence
Sex linkage
Stem cell research
Stem cells
Studies
Surface markers
Tumors
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Summary:Mesenchymal stem cells (MSCs) are a mixture of progenitors that are heterogeneous in their regenerative potential. Development of MSC therapies with consistent efficacy is hindered by the absence of an immunophenotype of MSC heterogeneity. This study evaluates decoy TRAIL receptor CD264 as potentially the first surface marker to detect cellular aging in heterogeneous MSC cultures. CD264 surface expression, regenerative potential, and metrics of cellular aging were assessed in vitro for marrow MSCs from 12 donors ages 20-60 years old. Male and female donors were age matched. Expression of CD264 was compared with that of p16, p21, and p53 during serial passage of MSCs. When CD264 cell content was 20% to 35%, MSC cultures from young (ages 20-40 years) and older (ages 45-60 years) donors proliferated rapidly and differentiated extensively. Older donor MSCs containing 
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-017-0649-4