Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Lactic acid bacteria species were molecularly identified in milk from Lacaune, Santa Inês and crossbred sheep breeds and their in vitro probiotic potential was evaluated. The species identified were Enterococcus faecium (56.25%), E. durans (31.25%) and E. casseliflavus (12.5%). No other lactic acid...

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Bibliographic Details
Published in:Arquivo brasileiro de medicina veterinária e zootecnia 2014-06, Vol.66 (3), p.940-948
Main Authors: Acurcio, L.B., Souza, M.R., Nunes, A.C., Oliveira, D.L.S., Sandes, S.H.C., Alvim, L.B.
Format: Article
Language:English
Subjects:
enterococos
leite de ovelha
potencial probiótico
VETERINARY SCIENCES
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Summary:Lactic acid bacteria species were molecularly identified in milk from Lacaune, Santa Inês and crossbred sheep breeds and their in vitro probiotic potential was evaluated. The species identified were Enterococcus faecium (56.25%), E. durans (31.25%) and E. casseliflavus (12.5%). No other lactic acid bacteria species, such as lactobacilli, was identified. Most of the isolated enterococci were resistant to gastric pH (2.0) and to 0.3% oxgall. All tested enterococci were resistant to ceftazidime, oxacillin and streptomycin and sensible to clindamycin, erythromycin and penicillin. The resistance to ciprofloxacin, gentamicin, tetracycline and vancomycin varied among tested species. All tested enterococci strongly inhibited (P
ISSN:0102-0935
1678-4162
0102-0935
DOI:10.1590/1678-41625796