Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed Gracilaria ornata

The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and the effect on bacterial growth of a crude sulfated polysaccharide (SP) from the red marine alga G. ornata (Go). Go-1 (25[degrees]C), Go-2 (80[degrees]C), and Go-3 (80[degree...

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Published in:Brazilian archives of biology and technology 2012-04, Vol.55 (2), p.171-181
Main Authors: Amorim, Rodrigo das Neves dos Santos, Rodrigues, José Ariévilo Gurgel, Holanda, Márjory Lima, Quinderé, Ana Luíza Gomes, Paula, Regina Célia Monteiro de, Melo, Vânia Maria Maciel, Benevides, Norma Maria Barros
Format: Article
Language:English
Subjects:
antimicrobial
Bacillus subtilis
chemical analysis
Enterobacter
Escherichia coli
Gracilaria
Pseudomonas aeruginosa
Rhodophyta
Staphylococcus aureus
sulfated macromolecules
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Summary:The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and the effect on bacterial growth of a crude sulfated polysaccharide (SP) from the red marine alga G. ornata (Go). Go-1 (25[degrees]C), Go-2 (80[degrees]C), and Go-3 (80[degrees]C) were sequentially extracted and yielded 9.2%. The contents of sulfate (5.88-10.3%) and proteins (0.1-3.7%) were small. The values of SR were [ mu ] sub(D) super(20[degrees]f) -19.0, -51.0, and -56.5, respectively. IR spectrums showed the presence of galactose-4 sulfate and absence of 3, 6-anydrogalactose-2 sulfate, galactose-6 sulfate and galactose-2 sulfate. SR and IR techniques confirmed SPs. Go-3 was tested on the growth of bacteria (Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogens, Escherichia coli, Pseudomonas aeruginosa, Salmonela choleraesuis and Salmonela typhi), but only E. coli was inhibited.
ISSN:1516-8913
1516-8913
1678-4324
DOI:10.1590/S1516-89132012000200001