Phytochemical and Biological Profile of Moricandia arvensis (L.) DC.: An Inhibitor of Pancreatic Lipase

Pancreatic lipase, a key enzyme for lipid absorption, is one of the most important targets for the treatment of obesity, while natural compounds have recently attracted much interest as potential inhibitors of this enzyme. Here, in an attempt to find new effective agents, the methanolic extract from...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2018-10, Vol.23 (11), p.2829
Main Authors: Marrelli, Mariangela, Morrone, Federica, Argentieri, Maria Pia, Gambacorta, Lucia, Conforti, Filomena, Avato, Pinarosa
Format: Article
Language:English
Subjects:
Absorption
Acetic acid
Aglycones
antioxidant
Antioxidants
Bleaching
Carotene
Dichloromethane
Ethyl acetate
Fatty acids
Flavone glycosides
Flavonoids
Flavonols
glucosinolates
Glycosides
Herbal medicine
Kaempferol
Linoleic acid
Lipase
Lipids
Metabolites
Moricandia arvensis
Obesity
Overweight
Pancreas
pancreatic lipase
Peroxidation
Phytochemicals
Quercetin
β-Carotene
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Summary:Pancreatic lipase, a key enzyme for lipid absorption, is one of the most important targets for the treatment of obesity, while natural compounds have recently attracted much interest as potential inhibitors of this enzyme. Here, in an attempt to find new effective agents, the methanolic extract from (L.) DC. and its sub-extracts were investigated for their potential inhibitory activity. The ability to inhibit pancreatic lipase was verified through the in vitro evaluation of the prevention of -nitrophenyl caprylate hydrolysis. The antioxidant activity was also verified by means of DPPH and β-carotene bleaching tests. Compositional profiling revealed that flavonoid glycosides were the main specialized metabolites present in the methanolic extract from the aerial parts of the plant with kaempferol and quercetin representing the two -glycosylated aglycones. Kaempferol-3- -β-(2″- -glucosyl)-rutinoside and kaempferol-3- - arabinosyl-7- -rhamnoside were the most abundant flavonols. The crude methanolic extract and the dichloromethane and ethyl acetate sub-extracts showed a strong lipase inhibitory activity, with IC values of 2.06 ± 0.02, 1.52 ± 0.02 and 1.31 ± 0.02 mg/mL, respectively. The best capacity to scavenge DPPH radical was detected for the ethyl acetate sub-extract (IC = 171.9 ± 1.0 µg/mL), which was also effective in protecting linoleic acid from peroxidation (IC = 35.69 ± 2.30 µg/mL). Obtained results support the hypothesis that can be a source of bioactive phytochemicals for the pharmacological inhibition of dietary lipids absorption.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23112829