Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence micros...

Full description

Saved in:
Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-12, Vol.11 (1), p.35
Main Authors: Hickey, Shane M, Ung, Ben, Bader, Christie, Brooks, Robert, Lazniewska, Joanna, Johnson, Ian R D, Sorvina, Alexandra, Logan, Jessica, Martini, Carmela, Moore, Courtney R, Karageorgos, Litsa, Sweetman, Martin J, Brooks, Douglas A
Format: Article
Language:English
Subjects:
Animals
cellular imaging
Fluorescence microscopy
Fluorescent Dyes - chemistry
Humans
imaging agents
Imaging, Three-Dimensional
Lasers
Light
Medical research
Micrography
Microscopes
Microscopy
microscopy techniques
Microscopy, Fluorescence
Models, Biological
Organelles - metabolism
Review
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells11010035