Protective effects of fentanyl preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion in rats

We aimed to study the effect of fentanyl (Fen) preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion (I/R) in rats. A total of 120 Sprague Dawley male rats (age: 3 months) were randomly divided into: sham operation group (S group), I/R group, normal saline I/R group (NS group),...

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Published in:Brazilian journal of medical and biological research 2017-01, Vol.50 (2), p.e5286-e5286
Main Authors: Xu, Q, Li, Q-G, Fan, G-R, Liu, Q-H, Mi, F-L, Liu, B
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
B-cell lymphoma 2
Bax
BIOLOGY
Biomedical Sciences
Dosage and administration
Drug therapy
Fentanyl
Fentanyl - therapeutic use
Heart cells
Hemodynamic parameters
Ischemia-reperfusion
Male
MEDICINE, RESEARCH & EXPERIMENTAL
Myocardial apoptosis
Myocardial infarction
Myocardial Reperfusion Injury - pathology
Myocardial Reperfusion Injury - prevention & control
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - pathology
Protective Agents - therapeutic use
Rats
Rats, Sprague-Dawley
Reperfusion injury
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Summary:We aimed to study the effect of fentanyl (Fen) preconditioning on cardiomyocyte apoptosis induced by ischemia-reperfusion (I/R) in rats. A total of 120 Sprague Dawley male rats (age: 3 months) were randomly divided into: sham operation group (S group), I/R group, normal saline I/R group (NS group), and fentanyl low, middle, and high dose groups (Fen1: 2 μg/kg; Fen2: 4 μg/kg; Fen3: 6 μg/kg). Heart rate (HR), mean arterial pressure (MAP), left ventricular developed pressure (LVDP), ±dp/dtmax, malondialdehyde (MDA), superoxide dismutase (SOD) activity, creatine phosphokinase-MB (CK-MB), and cardiac troponin-I (cTnI) were measured. Myocardial ischemic (MI) area, total apoptotic myocardial cells, and protein and mRNA expressions of B-cell lymphoma 2 (Bcl-2) and Bax were detected. HR and MAP were higher, while LVDP and ±dp/dtmax were close to the base value in the Fen groups compared to those in the I/R group. Decreased MDA concentration and CK-MB value and increased SOD activity were found in the Fen groups compared to the I/R group, while cTnI concentration was significantly lower in the Fen1 and Fen2 groups (all P
ISSN:0100-879X
1414-431X
1414-431X
DOI:10.1590/1414-431X20165286