Leukocyte Esterase Strip Quantitative Detection Based on RGB Photometry is a Probable Method to Diagnose Periprosthetic Joint Infection: An Exploratory Study

Objective Leucocyte esterase (LE) strip test is the most rapid, convenient, and cheap method to diagnose chronic periprosthesis joint infection (PJI). However, the determination of LE strip mainly relies on colorimetric method with strong subjectivity, which leads to low diagnostic accuracy. Therefo...

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Bibliographic Details
Published in:Orthopaedic surgery 2023-04, Vol.15 (4), p.983-992
Main Authors: Zheng, Qing‐yuan, Ren, Peng, Cheng, Long, Liu, Hao, Zhao, Runkai, Lv, Yicun, Geng, Zongjie, Lu, Kuan, Ni, Ming, Zhang, Guo‐qiang
Format: Article
Language:English
Subjects:
Blood
Body mass index
Chi-square test
Chronic Periprosthetic Joint Infection
Clinical
Immune system
Joint replacement surgery
Knee
LE Strip Test
Leukocytes
Medical laboratories
Patients
Quantitative Detection
Regression analysis
RGB Photometry
Urinary tract diseases
Urinary tract infections
Urogenital system
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Summary:Objective Leucocyte esterase (LE) strip test is the most rapid, convenient, and cheap method to diagnose chronic periprosthesis joint infection (PJI). However, the determination of LE strip mainly relies on colorimetric method with strong subjectivity, which leads to low diagnostic accuracy. Therefore, we try to convert LE strip images into digital data through the RGB photometric system to achieve objective diagnosis. This method will greatly improve the accuracy of LE strip detection and diagnosis of PJI. Methods From January 2021 to September 2021, 46 patients with suspected PJI after total hip and knee arthroplasty underwent diagnostic joint puncture. After effective joint fluid samples were harvested, they were divided into original fluid and centrifuged fluid for LE strip detection. Real‐time images of LE strip were taken at 90 s, 3 min, 5 min, 10 min, and 15 min after sampling, and their brightness (Y) was obtained after they were input into an RGB photometric system. Grouping was based on centrifugation, infection, and time points, and then the differences in brightness among groups were compared. The correlation between LE strip image brightness and WBC count was evaluated. Student t‐test was used for the parametric data and chi‐square test for qualitative data. Simple linear regression was utilized to analyze the correlation between brightness and WBC count in each group. Results Included were 19 cases of PJI and 27 Non‐PJI subjects diagnosed against ICM2018 diagnostic criteria. The brightness was lower in the PJI group than in Non‐PJI group (p 
ISSN:1757-7853
1757-7861
DOI:10.1111/os.13667