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The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group)
f. sp. ( ) causes basal rot disease in species, including onions ( L.) and shallots ( L. Aggregatum group). Among species, shallots can be crossbred with onions and are relatively more resistant to than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However,...
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| Published in: | Microorganisms (Basel) 2023-11, Vol.11 (12), p.2861 |
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| Main Authors: | , , , , , |
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| container_issue | 12 |
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| container_title | Microorganisms (Basel) |
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| creator | Sakane, Kosei Kunimoto, Masaaki Furumoto, Kazuki Shigyo, Masayoshi Sasaki, Kazunori Ito, Shin-Ichi |
| description | f. sp.
(
) causes basal rot disease in
species, including onions (
L.) and shallots (
L. Aggregatum group). Among
species, shallots can be crossbred with onions and are relatively more resistant to
than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However, the mechanisms underlying the molecular interactions between shallots and
remain unclear. This study demonstrated that SIX5, an effector derived from
(FocSIX5), acts as an avirulence effector in shallots. We achieved this by generating a
gene knockout mutant in
for which experiments which revealed that it caused more severe wilt symptoms in
-resistant shallots than the wild-type
and
gene complementation mutants. Moreover, we demonstrated that a single amino acid substitution (R67K) in FocSIX5 was insufficient to overcome shallot resistance to
. |
| doi_str_mv | 10.3390/microorganisms11122861 |
| format | article |
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(
) causes basal rot disease in
species, including onions (
L.) and shallots (
L. Aggregatum group). Among
species, shallots can be crossbred with onions and are relatively more resistant to
than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However, the mechanisms underlying the molecular interactions between shallots and
remain unclear. This study demonstrated that SIX5, an effector derived from
(FocSIX5), acts as an avirulence effector in shallots. We achieved this by generating a
gene knockout mutant in
for which experiments which revealed that it caused more severe wilt symptoms in
-resistant shallots than the wild-type
and
gene complementation mutants. Moreover, we demonstrated that a single amino acid substitution (R67K) in FocSIX5 was insufficient to overcome shallot resistance to
.</description><identifier>ISSN: 2076-2607</identifier><identifier>EISSN: 2076-2607</identifier><identifier>DOI: 10.3390/microorganisms11122861</identifier><identifier>PMID: 38138005</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Allium ; Allium cepa ; Amino acid substitution ; Amino acids ; Basal rot ; Complementation ; Fusarium oxysporum ; Fusarium oxysporum f. sp. cepae ; Genes ; Genetic engineering ; Leaves ; Molecular interactions ; Mutants ; Onions ; Pathogens ; Peptides ; Polymerase chain reaction ; Proteins ; shallot ; Signs and symptoms ; SIX gene family ; SIX5 ; Tomatoes ; Vegetables ; Virulence ; virulence effector ; Wilt</subject><ispartof>Microorganisms (Basel), 2023-11, Vol.11 (12), p.2861</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c400t-b7564d0beb614f990d91ee1310b7df31edd8fb88d1ee770ff7a7769f5a49c5a73</cites><orcidid>0000-0003-4014-6613 ; 0000-0002-4520-0378</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2904851864/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2904851864?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25752,27923,27924,37011,37012,44589,74997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38138005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakane, Kosei</creatorcontrib><creatorcontrib>Kunimoto, Masaaki</creatorcontrib><creatorcontrib>Furumoto, Kazuki</creatorcontrib><creatorcontrib>Shigyo, Masayoshi</creatorcontrib><creatorcontrib>Sasaki, Kazunori</creatorcontrib><creatorcontrib>Ito, Shin-Ichi</creatorcontrib><title>The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group)</title><title>Microorganisms (Basel)</title><addtitle>Microorganisms</addtitle><description>f. sp.
(
) causes basal rot disease in
species, including onions (
L.) and shallots (
L. Aggregatum group). Among
species, shallots can be crossbred with onions and are relatively more resistant to
than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However, the mechanisms underlying the molecular interactions between shallots and
remain unclear. This study demonstrated that SIX5, an effector derived from
(FocSIX5), acts as an avirulence effector in shallots. We achieved this by generating a
gene knockout mutant in
for which experiments which revealed that it caused more severe wilt symptoms in
-resistant shallots than the wild-type
and
gene complementation mutants. Moreover, we demonstrated that a single amino acid substitution (R67K) in FocSIX5 was insufficient to overcome shallot resistance to
.</description><subject>Allium</subject><subject>Allium cepa</subject><subject>Amino acid substitution</subject><subject>Amino acids</subject><subject>Basal rot</subject><subject>Complementation</subject><subject>Fusarium oxysporum</subject><subject>Fusarium oxysporum f. sp. cepae</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Leaves</subject><subject>Molecular interactions</subject><subject>Mutants</subject><subject>Onions</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>shallot</subject><subject>Signs and symptoms</subject><subject>SIX gene family</subject><subject>SIX5</subject><subject>Tomatoes</subject><subject>Vegetables</subject><subject>Virulence</subject><subject>virulence effector</subject><subject>Wilt</subject><issn>2076-2607</issn><issn>2076-2607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptUcFq3DAUNKWlCWl-IQh6SQ-7lSxLso4mJOnCQgtJoTfzLD1ttNiWK9ltc--HV9tNQykVDzQMM8OTpiguGF1zrun7wZsYQtzB6NOQGGNlWUv2ojgtqZKrUlL18i98UpyntKf5aMZrwV4XJ7zOiFJxWvy8f0Byt_kiyKcYZvQjyXOzJIh-GUj48ZimEDNya5KmNTE4AZLGzIlAnpE033xcehwNkmvn0Mwhkjl8h2jJ3QP0fZjJJWn6_pB2MJPtmjS7XcQdzJm6jWGZ3r0pXjnoE54_3WfF55vr-6sPq-3H281Vs12ZitJ51SkhK0s77CSrnNbUaobIOKOdso4ztLZ2XV3bzCpFnVOglNROQKWNAMXPis0x1wbYt1P0A8THNoBvfxP5R1uIszc9tqVESzXnBipVgdPgDOcMOkEzwa3JWZfHrCmGrwumuR18Mtj3MGJYUltqKkTuRZRZ-vYf6T4sccwvPaiqXEktq6ySR1XuNqWI7nlBRttD7e3_a8_Gi6f4pRvQPtv-lMx_AY3Sq_w</recordid><startdate>20231126</startdate><enddate>20231126</enddate><creator>Sakane, Kosei</creator><creator>Kunimoto, Masaaki</creator><creator>Furumoto, Kazuki</creator><creator>Shigyo, Masayoshi</creator><creator>Sasaki, Kazunori</creator><creator>Ito, Shin-Ichi</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-4014-6613</orcidid><orcidid>https://orcid.org/0000-0002-4520-0378</orcidid></search><sort><creationdate>20231126</creationdate><title>The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group)</title><author>Sakane, Kosei ; Kunimoto, Masaaki ; Furumoto, Kazuki ; Shigyo, Masayoshi ; Sasaki, Kazunori ; Ito, Shin-Ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-b7564d0beb614f990d91ee1310b7df31edd8fb88d1ee770ff7a7769f5a49c5a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Allium</topic><topic>Allium cepa</topic><topic>Amino acid substitution</topic><topic>Amino acids</topic><topic>Basal rot</topic><topic>Complementation</topic><topic>Fusarium oxysporum</topic><topic>Fusarium oxysporum f. sp. cepae</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Leaves</topic><topic>Molecular interactions</topic><topic>Mutants</topic><topic>Onions</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>shallot</topic><topic>Signs and symptoms</topic><topic>SIX gene family</topic><topic>SIX5</topic><topic>Tomatoes</topic><topic>Vegetables</topic><topic>Virulence</topic><topic>virulence effector</topic><topic>Wilt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakane, Kosei</creatorcontrib><creatorcontrib>Kunimoto, Masaaki</creatorcontrib><creatorcontrib>Furumoto, Kazuki</creatorcontrib><creatorcontrib>Shigyo, Masayoshi</creatorcontrib><creatorcontrib>Sasaki, Kazunori</creatorcontrib><creatorcontrib>Ito, Shin-Ichi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Microorganisms (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakane, Kosei</au><au>Kunimoto, Masaaki</au><au>Furumoto, Kazuki</au><au>Shigyo, Masayoshi</au><au>Sasaki, Kazunori</au><au>Ito, Shin-Ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group)</atitle><jtitle>Microorganisms (Basel)</jtitle><addtitle>Microorganisms</addtitle><date>2023-11-26</date><risdate>2023</risdate><volume>11</volume><issue>12</issue><spage>2861</spage><pages>2861-</pages><issn>2076-2607</issn><eissn>2076-2607</eissn><abstract>f. sp.
(
) causes basal rot disease in
species, including onions (
L.) and shallots (
L. Aggregatum group). Among
species, shallots can be crossbred with onions and are relatively more resistant to
than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However, the mechanisms underlying the molecular interactions between shallots and
remain unclear. This study demonstrated that SIX5, an effector derived from
(FocSIX5), acts as an avirulence effector in shallots. We achieved this by generating a
gene knockout mutant in
for which experiments which revealed that it caused more severe wilt symptoms in
-resistant shallots than the wild-type
and
gene complementation mutants. Moreover, we demonstrated that a single amino acid substitution (R67K) in FocSIX5 was insufficient to overcome shallot resistance to
.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38138005</pmid><doi>10.3390/microorganisms11122861</doi><orcidid>https://orcid.org/0000-0003-4014-6613</orcidid><orcidid>https://orcid.org/0000-0002-4520-0378</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2076-2607 |
| ispartof | Microorganisms (Basel), 2023-11, Vol.11 (12), p.2861 |
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| recordid | cdi_doaj_primary_oai_doaj_org_article_26ed0933ca474af9afc331ab50a473dc |
| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Allium Allium cepa Amino acid substitution Amino acids Basal rot Complementation Fusarium oxysporum Fusarium oxysporum f. sp. cepae Genes Genetic engineering Leaves Molecular interactions Mutants Onions Pathogens Peptides Polymerase chain reaction Proteins shallot Signs and symptoms SIX gene family SIX5 Tomatoes Vegetables Virulence virulence effector Wilt |
| title | The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group) |
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