Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS

As a result of the capability of fungi to respond to culture conditions, we aimed to explore and compare the antibacterial activity and chemical diversity of two endophytic fungi isolated from Hyptis dilatata and cultured under different conditions by the addition of chemical elicitors, changes in t...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2020-09, Vol.6 (3), p.140
Main Authors: Aguilar-Perez, Madelaine M, Torres-Mendoza, Daniel, Vasquez, Roger, Rios, Nivia, Cubilla-Rios, Luis
Format: Article
Language:English
Subjects:
Antibacterial activity
Antibiotics
Ascomycota
Cancer
chemical elicitors
Chemical properties
Chemicals
Endophytes
Endophytic fungi
Fungi
Hyptis dilatata
Liquid chromatography
Listeria
Mass spectroscopy
Metabolites
Methods
Microorganisms
Minimum inhibitory concentration
Natural products
Pestalotiopsis
Pestalotiopsis mangiferae
Pestalotiopsis microspora
Pneumonia
Protopulvinaria mangiferae
R&D
Research & development
Time-of-flight mass spectrometry
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Summary:As a result of the capability of fungi to respond to culture conditions, we aimed to explore and compare the antibacterial activity and chemical diversity of two endophytic fungi isolated from Hyptis dilatata and cultured under different conditions by the addition of chemical elicitors, changes in the pH, and different incubation temperatures. Seventeen extracts were obtained from both Pestalotiopsis mangiferae (man-1 to man-17) and Pestalotiopsis microspora (mic-1 to mic-17) and were tested against a panel of pathogenic bacteria. Seven extracts from P. mangiferae and four extracts from P. microspora showed antibacterial activity; while some of these extracts displayed a high-level of selectivity and a broad-spectrum of activity, Pseudomonas aeruginosa was the most inhibited microorganism and was selected to determine the minimal inhibitory concentration (MIC). The MIC was determined for extracts man-6 (0.11 μg/mL) and mic-9 (0.56 μg/mL). Three active extracts obtained from P. mangiferae were analyzed by Liquid Chromatography-Electrospray Ionization-Quadrupole-Time of Flight-Mass Spectrometry (LC–ESI–Q–TOF–MS) to explore the chemical diversity and the variations in the composition. This allows us to propose structures for some of the determined molecular formulas, including the previously reported mangiferaelactone (1), an antibacterial compound.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof6030140