ER assembly of SNARE complexes mediating formation of partitioning membrane in Arabidopsis cytokinesis

Intracellular membrane fusion mediates diverse processes including cell growth, division and communication. Fusion involves complex formation between SNARE proteins anchored to adjacent membranes. How and in what form interacting SNARE proteins reach their sites of action is virtually unknown. We ha...

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Bibliographic Details
Published in:eLife 2017-05, Vol.6
Main Authors: Karnahl, Matthias, Park, Misoon, Mayer, Ulrike, Hiller, Ulrike, Jürgens, Gerd
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - physiology
Cell Biology
Cell division
Cell interactions
Cell Membrane - metabolism
Cell membranes
Cytokinesis
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
Golgi cells
Intracellular signalling
Membrane fusion
membrane traffic
Membrane vesicles
Molecular biology
Mutation
Observations
Physiological aspects
Plant Biology
Protein Transport
Proteins
Short Report
SNAP receptors
SNARE complex
SNARE Proteins - metabolism
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Summary:Intracellular membrane fusion mediates diverse processes including cell growth, division and communication. Fusion involves complex formation between SNARE proteins anchored to adjacent membranes. How and in what form interacting SNARE proteins reach their sites of action is virtually unknown. We have addressed this problem in the context of plant cell division in which a large number of TGN-derived membrane vesicles fuse with one another to form the partitioning membrane. Blocking vesicle formation at the TGN revealed -SNARE complexes. These inactive cytokinetic SNARE complexes were already assembled at the endoplasmic reticulum and, after passage through Golgi/TGN to the cell division plane, transformed into fusogenic SNARE complexes. This mode of trafficking might ensure delivery of large stoichiometric quantities of SNARE proteins required for forming the partitioning membrane in the narrow time frame of plant cytokinesis. Such long-distance trafficking of inactive SNARE complexes would also facilitate directional growth processes during cell differentiation.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.25327