The functional and molecular studies on involvement of hydrogen sulphide in myometrial activity of non-pregnant buffaloes (Bubalus bubalis)

Hydrogen sulphide (H S), a member of the gasotransmitters family, is known to play patho-physiological role in different body systems including during pregnancy. But its involvement in myometrial spontaneity and associated signalling pathways in uterus in non-pregnant animals is yet to be studied. P...

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Published in:BMC veterinary research 2017-12, Vol.13 (1), p.379-7, Article 379
Main Authors: Nair, Sooraj V, Sharma, Vipin, Sharma, Abhishek, Nakade, Udayraj P, Jaitley, Pooja, Mathesh, Karikalan, Choudhury, Soumen, Garg, Satish Kumar
Format: Article
Language:English
Subjects:
Antibodies
Calcium channels
Cysteine
Cystine
Enzymes
H2S
Hydrogen
Hydrogen sulfide
Immunohistochemistry
L-Cysteine
L-type Ca2+ channel
Myometrium
Nifedipine
Non-pregnant buffalo
Physiological aspects
Pregnancy
Pregnant women
Thiols
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Summary:Hydrogen sulphide (H S), a member of the gasotransmitters family, is known to play patho-physiological role in different body systems including during pregnancy. But its involvement in myometrial spontaneity and associated signalling pathways in uterus in non-pregnant animals is yet to be studied. Present study describes the effect of L-cysteine, an endogenous H S donor, on isolated myometrial strips of non-pregnant buffaloes and the underlying signaling mechanism(s). L-cysteine (10 nM-30 mM) produced concentration-dependent contractile effect on buffalo myometrium which was extracellular Ca and L-type calcium channels-dependent. Significant rightward shift of dose-response curve of L-cysteine was observed with significant decrease in maxima in the presence of amino-oxyacetic acid (AOAA; 100 μM) and d, l-propargylglycine (PAG; 100 μM), the specific blockers of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE), respectively. Existence of CBS enzyme of 63 kDa and CSE of 45 kDa molecular weights was confirmed by western blot using specific antibodies and also by immunohistochemistry. Endogenous H S along with its biosynthetic enzymes (CBS and CSE) is evidently present in uteri of non-pregnant buffaloes and it regulates spontaneity in uteri of non-pregnant buffaloes and this effect is dependent on extracellular Ca influx through nifedipine-sensitive L-type calcium channels. Thus H S-signalling pathway may be a potential target to alter the uterine activities in physiology and patho-physiolgical states.
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-017-1288-9