Detection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective study

The use of one-step nucleic acid amplification (OSNA) allows for lymph node (LN) metastasis to be detected rapidly and accurately. We conducted a prospective single-centre clinical trial to evaluate OSNA assay in detecting LN metastasis of lung cancer. A total of 705 LNs from 160 patients with clini...

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Bibliographic Details
Published in:Journal of translational medicine 2019-07, Vol.17 (1), p.233-233, Article 233
Main Authors: Escalante Pérez, María, Hermida Romero, María Teresa, Otero Alén, Begoña, Álvarez Martínez, Mónica, Fernández Prado, Ricardo, de la Torre Bravos, Mercedes, Concha López, Ángel
Format: Article
Language:English
Subjects:
Cancer metastasis
Cancer patients
Cancer research
Clinical trials
Diagnosis
Gene amplification
Genetic aspects
Health aspects
Intraoperative
Keratin
Keratin 19
LN status
Lung cancer
Lymph nodes
Medical examination
Metastasis
OSNA assay
Surgery
Tumors
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Summary:The use of one-step nucleic acid amplification (OSNA) allows for lymph node (LN) metastasis to be detected rapidly and accurately. We conducted a prospective single-centre clinical trial to evaluate OSNA assay in detecting LN metastasis of lung cancer. A total of 705 LNs from 160 patients with clinical stage IA to IVA lung cancer were included in this study. The LNs were divided and submitted to routine histological diagnosis and OSNA assay and the results were compared. We also examined keratin 19 expression of different histological types lung primary tumours. When the cut-off value was set to 250 copies/µl, the concordance rate between the two methods was 96.17% and the sensitivity 97.14%. Discordant results were observed in 27 LNs of 21 patients. Most of these discordant results were molecular micrometastasis expressing a very low number of copies with negative histology. Most thoracic tumours were positive for keratin 19. Our data show that the OSNA assay might be a useful and sensitive method to diagnose LN metastasis in lung cancer and could be applied to intraoperative decision-making in personalised lung cancer surgery based on LN status and a more accurate staging of patients.
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-019-1974-4